| 17826976 |
Istamboulie G, Andreescu S, Marty JL, Noguer T: Highly sensitive detection of organophosphorus insecticides using magnetic microbeads and genetically engineered acetylcholinesterase. Biosens Bioelectron. 2007 Nov 30;23(4):506-12. Epub 2007 Aug 2.
This work presents a biosensor for organophosphorus pesticides based on immobilisation of a highly sensitive genetically engineered acetylcholinesterase (B394) by affinity interactions on metal chelate-functionalised magnetic microbeads. The developed sensor has been compared with those based on the widely used Electric eel cholinesterase and a classical entrapment procedure in a polyvinylalcohol-based matrix. The use of the B394 enzyme allowed lowering both IC50 and LOD by a factor of 100 when compared with Electric eel enzyme sensor. The oriented and site-specific immobilisation combined with the high specificity of the B349 mutant allows a more sensitive detection of insecticides, concentrations as low as 1.31 (-11) M (IC10) being detected for both pesticides chlorpyriphos-oxon and chlorfenvinphos. |
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