| 16111029 |
Zhao HT, Zhu JB, Zhu YF, Wang Z, Ma X, Zhang T: [Establishment of reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells]. Wei Sheng Yan Jiu. 2005 May;34(3):281-4.
OBJECTIVE: Develop reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells, explore the antiandrogenic effects of 2,4-DDT and methoxychlor. METHODS: Chinese Ovary cells were cotransfected with the human androgen receptor expression vector, mouse mammary tumour virus MMTV-luciferase vector and phRL-SV40 using the transfection reagent Sofast, test the luciferase activity. After treatment of the cells for 24 h with dihydrotestosterone (DHT), flutamide (FLU), validate the effectiveness and specificity, also test the effect of 2,4-DDT and methoxychlor (METH). RESULTS: DHT is a potent agonist, 1 x 10 (-10) mol/L DHT could result in the enhancement of luciferase activity, FLU is a complete antagonist, that confirmed the effectiveness and specificity of test system. 2,4-DDT and METH are partial antagonists. 3 x 10 (-7) mol/L and above 2,4-DDT and METH could inhibit the androgenic activity of DHT. CONCLUSION: The assay we developed is doful, 2,4-DDT and METH could antagonize the androgenic effects. |
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