| 15642635 |
Rouhani Rankouhi T, Sanderson JT, van Holsteijn I, van Kooten P, Bosveld AT, van den Berg M: Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria). Aquat Toxicol. 2005 Jan 18;71(1):97-101. Epub 2004 Nov 19.
The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximately ethynylestradiol (EC50: 13-80 nM)> estrone (EC50: 218-241 nM)> DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 microM did not have any effect on in vitro vitellogenesis. |
1(0,0,0,1) |