| 18288358 |
Ye HY, Yin M, Shang YJ, Dai XD, Zhang SQ, Jing W, DU HQ, Zhang L, Pan J: [Differential expressions of lipid metabolism related genes in the liver of young apoE knockout mice.]. Sheng Li Xue Bao. 2008 Feb 25;60(1):51-8.
The work was aimed to investigate the differential expressions of lipid metabolism related genes in the early stage of atherosclerosis in the young apolipoprotein E deficient (apoE (-/-)) mice at different ages with normal chow diet. The genotypes of mice were identified by using multiplex polymerase chain reaction (multi-PCR) analysis. The semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR were used to analyze the expressions of lipid metabolism related genes in the liver of apoE (-/-) and age-matched wild type (WT) mice of 14-day old, 1-month old, 2-month old, 3-month old. The serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) contents were assayed using COD-PAP and GPO-PAP methods. The serum apolipoprotein B100 (apoB100) content was quantitated by immune turbidimetry. The hearts were perfusion-fixed in 4% formaldehyde, infiltrated with 30% gum sucrose for 24 h at 4 degrees C, and embedded in OCT compound. The aortic sinus tissues were serially sectioned at -15 degrees C, stained with Sudan IV, and counterstained with light green. The results were shown as follows. Compared with that in WT mice, the mRNA levels of apoA I and apoA IV in apoE (-/-) mice aged from 14-day old to 3-month old changed prominently (P <0.05), with apoA I up-regulated and apoA IV down-regulated. At the age of 1 month, the expression of apoB100 in apoE (-/-) mice was higher than that in WT mice (P <0.05). The expression of apoA V was up-regulated (P <0.05) and there was obvious lipid deposition in the aortic intima in apoE (-/-) mice at the age of 2 months. The expressions of fatty acid translocase (Fat/CD36) and angiopoietin-like protein 3 (Angptl 3) in apoE (-/-) mice were higher than those in WT mice at the age of 3 months (P <0.05), while the expressions of peroxisome proliferator-activated receptor alpha (PPARalpha), liver X receptor alpha (LXRalpha), carnitine palmitoyl transferase I (CPT I) and acyl coenzyme A oxidase 1 (ACOX1) showed no significant changes. The serum TC, TG, LDL-C and HDL-C contents in apoE (-/-) mice aged from 14-day old to 3-month old were higher than those in age-matched WT mice. apoE (-/-) mice showed a marked increase in serum apoB100 content, consistent with the trend of serum LDL-C content and apoB100 mRNA content in the liver. The results suggest that the mRNA expressions of apoA I, apoA IV, apoA V, apoB100 and Angptl 3 in apoE (-/-) mice change significantly compared with those in WT mice, and these genes might be relevant to the complicated lipid metabolism network, and involved in the early stage of atherogenesis. |
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