| 16325265 |
Sylte MJ, Kuckleburg CJ, Leite FP, Inzana TJ, Czuprynski CJ: Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells. Vet Immunol Immunopathol. 2006 Apr 15;110(3-4):303-9. Epub 2005 Dec 1.
Haemophilus somnus lipooligosaccharide (LOS)-induced apoptosis of bovine pulmonary artery endothelial cells has been shown previously to be dependent on caspase-8 activation. Activation of caspase-8 can occur via a death receptor-dependent mechanism (e.g., TNF-alpha binding to TNF-alpha receptor 1 (TNF-R1)). In this study, we tested the hypothesis that TNF-alpha can enhance LOS-induced apoptosis of bovine endothelial cells. Addition of exogenous recombinant human TNF-alpha alone failed to cause apoptosis, or enhance LOS-induced apoptosis, of bovine endothelial cells. However, blocking de novo protein synthesis by addition of cycloheximide significantly enhanced apoptosis of bovine endothelial cells by TNF-alpha, LOS or TNF-alpha and LOS in combination. Conversely, addition of soluble recombinant human (sTNF-R1) diminished LOS-induced apoptosis. Overall, these data suggest that LOS-mediated apoptosis may be due, in part, to activation of a TNR-R1-dependent death pathway. |
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