| 16466815 |
Aluru N, Vijayan MM: Resveratrol affects CYP1A expression in rainbow trout hepatocytes. . Aquat Toxicol. 2006 May 10;77(3):291-7. Epub 2006 Feb 8.
Resveratrol (RVT), a polyphenolic phytoalexin found in a variety of food products, including grapes and peanuts, is a well-characterized aryl hydrocarbon receptor (AhR) antagonist in mammalian cell lines. The lack of a reliable tool to block AhR signaling in rainbow trout (Oncorhynchus mykiss) prompted this study to evaluate the utility of RVT as an AhR antagonist in a piscine system. Trout hepatocytes in primary culture were exposed to varying doses of RVT (10 (-5)-10 (-12) M) either in the presence or absence of a well-established AhR agonist, beta-naphthoflavone (BNF). Indeed, BNF significantly elevated CYP1A and AhR protein expression in trout hepatocytes, and this response was inhibited at high (10 (-5)-10 (-7) M), but not low (10 (-8)-10 (-10) M), RVT doses. However, RVT alone at low doses (10 (-8)-10 (-10) M) significantly elevated CYP1A protein expression compared to control hepatocytes. This higher protein response with RVT was completely abolished with actinomycin D and cycloheximide suggesting transcriptional and translational regulation of CYP1A induction. Also, RVT at low (10 (-9) M), but not high (10 (-5) M), dose significantly elevated CYP1A1 mRNA transcript levels in BNF-primed hepatocytes supporting transcriptional modulation. Taken together, RVT is an effective AhR antagonist, but induces CYP1A expression at lower doses (<10 (-8) M) in trout hepatocytes. |
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