| 19667482 |
Saito K, Matsunaga H, Ohmura A, Takekuma M, Matsuki Y, Nakazawa H: Highly sensitive reporter gene assay for dioxins in human plasma by using cycloheximide as an enhancer substance. Anal Sci. 2009 Aug;25(8):1029-32.
A reporter gene assay (RGA) that uses a mouse liver recombinant Hepa1c1c7 containing the firefly luciferase gene was developed to screen for dioxins in human plasma. For a high-sensitivity method, the addition of cycloheximide to the culture medium brought about a fivefold increase in the sensitivity. The detection limit was 0.1 pg/microL/well. Aryl hydrocarbon receptor (AhR) binding affinity factors (AhR-BAF), calculated from the effect concentration 50 (EC (50)) value, showed approximately the same values as those in WHO-TEF (2006). A significant correlation between RGA and the conventional gas chromatography/mass spectrometry (GC/MS) method was obtained. |
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