| 12372564 |
Li H, Kang JF, Li YQ: Serotonin potentiation of glycine-activated whole-cell currents in the superficial laminae neurons of the rat spinal dorsal horn is mediated by protein kinase C. Brain Res Bull. 2002 Sep 30;58(6):593-600.
The modulatory effects of serotonin (5-HT) on glycine (Gly)-activated whole-cell currents were investigated in neurons acutely dissociated from the superficial laminae (I and II) of the rat spinal dorsal horn using the nystatin-perforated patch recording configuration under voltage-clamp conditions. Our results demonstrate that (1). Gly acted on strychnine (STR)-sensitive Gly receptors and elicited inward Cl (-) currents (I (Gly)) at a holding potential of -40 mV; (2). 5-HT potentiated I (Gly) without affecting the reversal potential of I (Gly); (3). the agonist (alpha-methyl-5-HT) and antagonist (ketanserine) of 5-HT (2) receptor mimicked and blocked the potentiating effect of 5-HT on I (Gly), respectively; (4). bisindolylmaleimide I (BIM), a selective inhibitor of protein kinase C (PKC), reduced the potentiating effect of 5-HT on I (Gly); and (5). 5-HT-induced enhancement of I (Gly) was not affected by pretreatment with 1,2-bis-(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxy-methyl) ester (BAPTA AM), a Ca (2+) chelator. These results indicate that (1). the potentiation of 5-HT on I (Gly) is mediated by 5-HT (2) receptor and through Ca (2+)-independent PKC intracellular signal transduction pathway; and (2). the interactions between 5-HT and Gly might modulate the transmission of nociceptive information through the spinal cord. |
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