| 12804573 |
Llanos MN, Ronco AM, Aguirre MC: Specific strychnine binding sites on acrosome-associated membranes of golden hamster spermatozoa. J Neurochem. 1996 Oct;67(4):1485-90.
This study demonstrates for the first time, that membrane vesicles originated from the hamster sperm head after the occurrence of the acrosome reaction, possess specific strychnine binding sites. [3H] Strychnine binding was saturable and reversible, being displaced by unlabeled strychnine (IC (50)=26.7+/-2.3 microM). Kinetic analysis revealed one binding site with K (d)=120nM and B (max)=142fmol/10 (6) spermatozoa. Glycine receptor agonists beta-alanine and taurine inhibited strychnine binding by 20-30%. Surprisingly, glycine stimulated binding by about 40-50%. Results obtained in this study strongly suggest the presence of glycine receptors-with distinctive kinetic properties on the periacrosomal plasma membrane of hamster spermatozoa. Localization of this receptor fits well with its previously proposed role in acrosomal exocytosis during mammalian fertilization. |
82(1,1,1,2) |