| 11395484 |
Breitinger HG, Villmann C, Becker K, Becker CM: Opposing effects of molecular volume and charge at the hyperekplexia site alpha 1 (P250) govern glycine receptor activation and desensitization. table of contents.
Allelic variants of the glycine receptor alpha1 subunit gene GLRA1 underlie the human neurological disorder hyperekplexia. Among these, the subunit variant alpha1 (P250T) is characterized by an amino acid substitution within the cytoplasmic TM1-2 loop. To identify structural elements at position alpha1 (250) that govern receptor function, homomeric mutant receptor channels were subjected to electrophysiological analysis after recombinant expression in HEK293 cells. Wild-type alpha1 (P250) channels were nondesensitizing with an EC (50) for glycine of 8 microm, whereas bulky hydrophobic side chains of the channel variants alpha1 (P250V/I/L/F) showed rapid desensitization (tau (desens), 50-250 ms) and EC (50) values of 400-1800 microm. Small side chains (P250G/A/S) gave rise to wild-type-like channels. Effects of volume were counteracted by charge: alpha1 (P250E/R) were nondesensitizing; EC (50) was approximately 70 microm. The mutants alpha1 (P250C/Y) displayed intermediate channel properties (EC (50), 42/70 microm; tau (desens), 3300/2800 ms, respectively). The isotropic forces volume and hydropathy were sufficient to account for the observed effects of residue alpha1 (250) on receptor function. Indeed, channel behavior was best predicted by a combined hydropathy/volume index describing the hydrophobic surface of individual amino acids. These observations characterize the short intracellular TM1-2 loop as a regulatory domain for channel activation and a crucial mediator of glycine receptor desensitization. |
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