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Jeong HJ, Lee JJ, Hahm ET, Han SH, Min BI, Cho YW: Role of protein kinase C in opioid modulation of glycine-gated Cl (-) current in rat periaqueductal gray neuron. Eur J Pharmacol. 2001 Nov 16;431(2):143-50.
The Role of protein kinase C in the modulatory effect of a mu-opioid receptor agonist, [D-Ala (2),N-Me-Phe (4),Gly (5)-ol] enkephalin (DAMGO), on the glycine-gated Cl (-) current was examined in acutely dissociated rat periaqueductal gray neurons. Using the nystatin-perforated patch-clamp technique, the neurons were voltage-clamped at -60 mV. The glycine-gated Cl (-) current (I (Gly)) was sensitive to strychnine. On pretreatment with 1 microM DAMGO, the 30-microM glycine response increased with time and showed a maximum amplitude of 209+/-37% of control. After a protein kinase C activator, phorbol-12-myristate-13-acetate (PMA, 0.1 microM) as pretreatment, I (Gly) increased to 138+/-6% of control. The DAMGO potentiation of I (Gly) was not altered by coapplication with PMA. Although protein kinase C inhibitors, chelerythrine (3 microM) and 2-[1-(3-dimethylaminopropyl) indol-3-yl]-3-(indol-3-yl) maleimide (GF109203X, 1 microM), did not alter I (Gly), the DAMGO-induced potentiation of I (Gly) was reduced to 161+/-21% or 164+/-31% of the control after coapplication with chelerythrine or GF109203X, respectively. These results indicate that the potentiation of I (Gly) by a mu-opioid receptor agonist is partly mediated by activation of protein kinase C. |
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