Protein Information

ID 4671
Name phospholipase C beta 2
Synonyms PLC beta 2; Phosphoinositide phospholipase C; PLCB 2; PLCB2; PLCB2 protein; Phospholipase C beta 2; PLCB2 proteins; Phospholipase C beta 2s

Compound Information

ID 336
Name strychnine
CAS strychnidin-10-one

Reference

PubMed Abstract RScore(About this table)
11245589 Yan W, Sunavala G, Rosenzweig S, Dasso M, Brand JG, Spielman AI: Bitter taste transduced by PLC-beta (2)-dependent rise in IP (3) and alpha-gustducin-dependent fall in cyclic nucleotides. Am J Physiol Cell Physiol. 2001 Apr;280(4):C742-51.
Current evidence points to the existence of multiple processes for bitter taste transduction. Previous work demonstrated involvement of the polyphosphoinositide system and an alpha-gustducin (Galpha (gust))-mediated stimulation of phosphodiesterase in bitter taste transduction. Additionally, a taste-enriched G protein gamma-subunit, Ggamma (13), colocalizes with Galpha (gust) and mediates the denatonium-stimulated production of inositol 1,4,5-trisphosphate (IP (3)). Using quench-flow techniques, we show here that the bitter stimuli, denatonium and strychnine, induce rapid (50-100 ms) and transient reductions in cAMP and cGMP and increases in IP (3) in murine taste tissue. This decrease of cyclic nucleotides is inhibited by Galpha (gust) antibodies, whereas the increase in IP (3) is not affected by antibodies to Galpha (gust). IP (3) production is inhibited by antibodies specific to phospholipase C-beta (2) (PLC-beta (2)), a PLC isoform known to be activated by Gbetagamma-subunits. Antibodies to PLC-beta (3) or to PLC-beta (4) were without effect. These data suggest a transduction mechanism for bitter taste involving the rapid and transient metabolism of dual second messenger systems, both mediated through a taste cell G protein, likely composed of Galpha (gust)/beta/gamma (13), with both systems being simultaneously activated in the same bitter-sensitive taste receptor cell.
1(0,0,0,1)