| 10841452 |
Wang DS, Xu TL, Li JS: 5-HT potentiates GABA- and glycine-activated chloride currents on the same neurons in rat spinal cord. J Hirnforsch. 1999;39(4):531-7.
The electrophysiological and pharmacological properties of GABA- and glycine (Gly)-induced responses were investigated in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using the nystatin perforated patch recording configuration under voltage-clamp conditions. At a holding potential of -40 mV, the currents evoked by GABA and Gly increased in a sigmoidal fashion with increasing agonist concentration. The reversal potentials of I (GABA) and I (Gly) were close to the Cl- equilibrium potential. Bicuculline (BIC) and strychnine (STR) suppressed I (GABA) and I (Gly) in a concentration-dependent manner, although they have different affinities for GABA and Gly. 5-HT potentiated both I (GABA) and I (Gly) via intracellular protein kinase C on the same neurons. The results indicated that the acutely dissociated SDCN neurons responded to both exogenous GABA and Gly, which activated GABA (A) and STR-sensitive Gly receptors, respectively, and 5-HT may produce spinal antinociception through postsynaptic potentiation of I (GABA) and I (Gly) when they are coreleased; GABA and Gly may act as cotransmitters implicated in the control of spinal nociceptive signal processing in the mammalian spinal cord. |
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