| 20017677 |
Zhu J, Zhang W, Li Y, Zhang W, Wang H, Zheng W, Wang C: ARMS test for diagnosis of CYP2C9 and VKORC1 mutation in patients with pulmonary embolism in Han Chinese. Pharmacogenomics. 2010 Jan;11(1):113-9.
AIMS: VKORC1 and CYP2C9 are important genetic factors affecting warfarin dose requirement. Our aim is to establish a simple, rapid and economical method to detect SNPs in the two genes. MATERIALS & METHODS: Primer Premier 5 was used and a normal primer, a mutational primer and a common primer have been designed using the amplification refractory mutation system for VKORC1 c.-1639G> A (rs9923231), CYP2C9*3 c.1075A> C (rs1057910) and CYP2C9*13 c.269T> C substitutions. The amplification refractory mutation system (ARMS) assay was validated by the restriction enzyme cleavage method of reference or direct sequencing. RESULTS: The ARMS primers designed can distinguish between heterozygotes and homozygotes successfully. In the Han Chinese population, patients with pulmonary embolism allele frequencies of CYP2C9*3 c.1075A> C (rs1057910), *13 c.269T> C and VKORC1 -1639G> A (rs9923231) are 4.3, 0.7 and 8.6%, respectively. CONCLUSION: The ARMS-PCR method is a simple, economical method that can be used for the rapid detection of SNPs in VKORC1 and CYP2C9. |
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