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Sabino BD, Torraca TG, Moura CM, Rozenbaum HF, de Castro Faria MV: Development of a Simple and Low-Cost Enzymatic Methodology for Quantitative Analysis of Carbamates in Meat Samples of Forensic Interest. J Forensic Sci. 2010 Mar 15.
Foods contaminated with a granulated material similar to Temik (a commercial pesticide formulation containing the carbamate insecticide aldicarb) are often involved in accidental ingestion, suicides, and homicides in Brazil. We developed a simple technique to detect aldicarb. This technique is based on the inhibition of a stable preparation of the enzyme acetylcholinesterase, and it is specially adapted for forensic purposes. It comprises an initial extraction step with the solvent methylene chloride followed by a colorimetric acetylcholinesterase assay. We propose that results of testing contaminated forensic samples be expressed in aldicarb equivalents because, even though all other carbamates are also potent enzyme inhibitors, aldicarb is the contaminant most frequently found in forensic samples. This method is rapid (several samples can be run in a period of 2 h) and low cost. This method also proved to be precise and accurate, detecting concentrations as low as 40 mug/kg of aldicarb in meat samples. |
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