Protein Information

Name casein
Synonyms Alpha; Alpha s1 casein; Alpha s1 casein precursor; CASA; CSN 1; CSN1; CSN1S1; Casein…

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
17289191 Medina P, Baresi L: Rapid identification of gelatin and casein hydrolysis using TCA. . J Microbiol Methods. 2007 May;69(2):391-3. Epub 2007 Jan 20.
18(0,0,3,3) Details
16401192 Chaudhry AS: Comparing two commercial enzymes to estimate in vitro proteolysis of purified or semi-purified proteins. J Anim Physiol Anim Nutr. 2005 Dec;89(11-12):403-12.


Experiment 2 compared the activity of 0.66 U of each of these enzymes per mg CP of semi-purified proteins including casein, wheat gluten (WG) and maize gluten meal (MG).
3(0,0,0,3) Details
12613855 Dave RI, McMahon DJ, Oberg CJ, Broadbent JR: Influence of coagulant level on proteolysis and functionality of mozzarella cheeses made using direct acidification. J Dairy Sci. 2003 Jan;86(1):114-26.


Changes in meltability, level of intact alpha (s1)-casein and beta-casein (by capillary electrophoresis), 12.5% TCA-soluble nitrogen, and complex modulus were measured.
1(0,0,0,1) Details
12670146 Sforza S, Ferroni L, Galaverna G, Dossena A, Marchelli R: Extraction, semi-quantification, and fast on-line identification of oligopeptides in Grana Padano cheese by HPLC-MS. J Agric Food Chem. 2003 Apr 9;51(8):2130-5.


A new method was developed based on the small fragmentation peaks arising from in-source fragmentation and from software analysis of the known casein sequences, which in many cases allowed the direct on-line identification of the oligopeptide sequences.
1(0,0,0,1) Details
19684562 Cupp-Enyard C: Use of the protease fluorescent detection kit to determine protease activity. J Vis Exp. 2009 Aug 4;(30). pii: 1514. doi: 10.3791/1514.


This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.
1(0,0,0,1) Details
15989231 Song SS, Ma H, Gao ZX, Jia ZH, Zhang X: [Construction of recombinant Escherichia coli strains producing poly (4-hydroxybutyric acid) homopolyester from glucose]. Wei Sheng Wu Xue Bao. 2005 Jun;45(3):382-6.


P (4HB) accumulation was enhanced up to 30% of cell dry weight, when cells were cultivated in mineral salts M9 medium plus glucose as sole carbon source with addition of yeast extract, tryptone, casein hydrolate into medium respectively.
1(0,0,0,1) Details
18429118 Carter N: Assays of protein kinases using exogenous substrates. . Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.7.


This unit describes assays for a variety of protein kinases that require different conditions to detect and measure their activities--cyclic nucleotide-dependent kinases, protein kinase C and isoforms, casein kinases, Ca (2+)/calmodulin-dependent kinases, and tyrosine kinase.
1(0,0,0,1) Details