Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name anthraquinone
CAS 9,10-anthracenedione

Reference List

PubMed Abstract RScore(About this table)
18804645 Hemmateenejad B, Shamsipur M, Safavi A, Sharghi H, Amiri AA: Reversed-phase high performance liquid chromatography (RP-HPLC) characteristics of some 9,10-anthraquinone derivatives using binary acetonitrile-water mixtures as mobile phase. 943.

The retention behavior of 28 synthesized 9,10-anthraquinone derivatives in a reversed-phase (RP) high performance liquid chromatography (HPLC) system has been studied on a C18-RP column using acetonitrile-water mixtures as mobile phase.
81(1,1,1,1) Details
18440751 Wang J, Li H, Jin C, Qu Y, Xiao X: Development and validation of a UPLC method for quality control of rhubarb-based medicine: fast simultaneous determination of five anthraquinone derivatives. J Pharm Biomed Anal. 2008 Aug 5;47(4-5):765-70. Epub 2008 Mar 20.

A reverse phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of five anthraquinone derivatives (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rhubarb using a Waters Acquity BEH C18, 50 mm x 2.1 mm, 1.7 microm column.
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14516002 Han YS, Hofte B, van der Heijden R, Verpoorte R: Analysis of anthraquinones in cell cultures of Cinchona 'Robusta' by HPLC with photodiode array and mass spectrometry detection. Talanta. 2008 Oct 19;77(1):351-9. Epub 2008 Jul 5.

Using a C18 column and gradient elution with a mobile phase system containing acetonitrile, water and trifluoroacetic acid, a satisfactory separation of both anthraquinone glycosides and aglycones in a crude dichloromethane extract could be obtained.
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20209948 Zhang C, Li L, Xiao Y, Lin N, Liu C, Tian G, Chen D: [Simultaneous determination of two anthraquinone-O-glucosides in five processed pieces from Rheum palamatum]. Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2872-5.

METHOD: The content of aleo-emodin-8-O-beta-D-glucoside, rhein-8-O-beta-D-glucoside were determined simultaneously by HPLC on a agilent TC-C18 (2) column at 35 degrees C with the mobile phase of acetonitrile-1% glacial acetic acid (20: 80).
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17553649 Shuangjin C, Fang F, Han L, Ming M: New method for high-performance liquid chromatographic determination of amantadine and its analogues in rat plasma. J Pharm Biomed Anal. 2007 Sep 3;44(5):1100-5. Epub 2007 Apr 24.

The derivatization was performed at room temperature using anthraquinone-2-sulfonyl chloride (ASC) as reagent for only 10 min and without postderivatization treatment to inactivate excess reagent.
The derivatives were analyzed by isocratic HPLC with a UV detector at 256 nm on a Lichrosper C18 column.
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16620517 Jiang JJ, Feng F, Ma M, Zhang ZX: Study on a new precolumn derivatization method in the determination of metformin hydrochloride. J Chromatogr Sci. 1978 Oct 10;16(10):496-9.

Metformin hydrochloride is successfully determined by reversed-phase high-performance liquid chromatography with a new precolumn derivatization method using 9,10-anthraquinone-2-sulfonyl chloride as the derivatization agent.
The derivatization product is analyzed on a Lichrosper C18 column (6.0 mm x 150 mm, 5 microm) at 256 nm with methanol-water (70:30, v/v) as the mobile phase.
1(0,0,0,1) Details
16862772 Chou PH, Matsui S, Matsuda T: Detection and identification of dyes showing AhR-binding affinity in treated sewage effluents. Water Sci Technol. 2006;53(11):35-42.

HPLC fractionation was carried out using C18 reversed-phase columns, and possible AhR ligands were further isolated and purified.
In addition, two other coloured ligands were postulated to be disperse anthraquinone dyes or their metabolites because of their UV spectra and HPLC retention times.
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19894533 Zhang C, Li L, Xiao Y, Lin N, Liu C, Li G, Pang Z, Chen D, Tian G: [Anthraquinone contents in five processed products from Rheum palamatum] . Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(15):1914-6.

METHOD: The contents of aleo-emodin, rhein, emodin, chrysophanol and physcion were determined simultaneously by HPLC on plogilent TC-C18 (2) column at 35 degrees C with the methanol-0.1% phosphoric acid (85: 15).
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16846714 Qu H, Ma Y, Yu K, Cheng Y: Simultaneous determination of eight active components in Chinese medicine 'YIQING' capsule using high-performance liquid chromatography. J Pharm Biomed Anal. 2007 Jan 4;43(1):66-72. Epub 2006 Jul 18.

The chromatographic separation was performed on a Lichrospher C18 column (250 mm x 4.6 mm i.d. with 5.0 microm particle size) with a simple linear gradient elution programme.
Due to the different UV characteristic of these components, three detection wavelengths were utilized for the quantitative analysis (UV wavelength 254 nm for anthraquinone derivatives, 278 nm for flavones compounds, and 345 nm for protoberberine alkaloids, respectively).
1(0,0,0,1) Details
730815 Komolafe OO: Ion-pair partition chromatography analysis of anthraquinone glycosides of senna and pharmaceutical preparations of senna. Phytochem Anal. 2003 Sep-Oct;14(5):298-305.

A Corasil C18 column (5 microns) with methyl alcohol (30 percent in water) containing 0.005 M tetrabutylammonium phosphate, pH 7.5, as mobile phase was used.
1(0,0,0,1) Details
17853381 Zahn M, Trinh T, Jeong ML, Wang D, Abeysinghe P, Jia Q, Ma W: A reversed-phase high-performance liquid chromatographic method for the determination of aloesin, aloeresin A and anthraquinone in Aloe ferox. J Chromatogr Sci. 2006 Apr;44(4):193-9.

The method utilized a C18 column with a water-methanol gradient and UV detection at 297 nm.
1(0,0,0,1) Details
16124606 Li XD, Huang LQ: [Influence of processing rhubarb on it's anthraquinone contents] . Zhongguo Zhong Yao Za Zhi. 2005 Jun;30(12):904-6

METHOD: Chromatographic conditions are as follow: Kromasil-C18 (4.6 mmx 150 mm, 5 microm) column, ethanol -H2O-H3PO4 (85:15:0.1) as mobile phase and the detection wavelength at 254 nm.
1(0,0,0,1) Details
17500359 Yi T, Leung KS, Lu GH, Zhang H, Chan K: Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum thunb by HPLC coupled with PAD and ESI/MS. Phytochem Anal. 2007 May-Jun;18(3):181-7.

Chromatographic separation was conducted on an Alltima C18 column using water:acetonitrile:acetic acid as the mobile phase.
Altogether nine compounds of various classes, including stilbene glucosides, anthraquinone glucosides and anthraquinone derivates, were identified by online ESI/MS.
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17766197 Liu H, Feng F, Ma M, Cui S, Xie D, Xu S: Pharmacokinetic study of three cardiovascular drugs by high-performance liquid chromatography using pre-column derivatization with 9,10-anthraquinone-2-sulfonyl chloride. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):42-8. Epub 2007 Aug 10.

The quantitative analysis was achieved using a C18 column at room temperature (25 degrees C), with various volume ratios of methanol-water as the mobile phase and a detection wavelength at 256 nm.
1(0,0,0,1) Details
17696360 Potterat O, Felten RV, Dalsgaard PW, Hamburger M: Identification of TLC markers and quantification by HPLC-MS of various constituents in noni fruit powder and commercial noni-derived products. J Agric Food Chem. 2007 Sep 5;55(18):7489-94. Epub 2007 Aug 14.

The anthraquinone alizarin, which has been reported from roots and leaves, was not detected in the samples investigated.
The separation was performed on a C18 column with a gradient of acetonitrile in water containing 0.1% formic acid.
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12682993 Feng F, Uno B, Goto M, Zhang ZX, An DK: Evaluation of anthraquinone-2-sulfonyl chloride for determination of phenol in water by liquid chromatography using pre-column phase-transfer catalysed derivatization. Phytochem Anal. 2008 Mar;19(2):122-6.

Combined with preconcentration using a Waters Sep-Pak Plus C18 cartridge, detection limits of phenols for water sample analysis were as low as 1 x 10 (-9) mol/L (ca. 0.1 microgram/L).
1(0,0,0,1) Details
19402184 Jiao Y, Zuo Y: Ultrasonic extraction and HPLC determination of anthraquinones, aloe-emodine, emodine, rheine, chrysophanol and physcione, in roots of Polygoni multiflori. Phytochem Anal. 2009 Jul;20(4):272-8.

INTRODUCTION: Polygoni multiflori, one of traditional Chinese herbal medicines for the treatment of various diseases commonly associated with aging, is known to contain active anthraquinone ingredients.
Separation and quantitation of anthraquinones was accomplished using a reversed-phase C18 column with the mobile phase of methanol-water-phosphoric acid (600:400:1), and the detection wavelength of 254 nm.
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