| Name | protein a |
|---|---|
| Synonyms | A1 activator; PSAP; Protein C; CSAct; Cerebroside sulfatase activator; Cerebroside sulfate activator; Co beta glucosidase; Component C… |
| Name | 1-naphthol |
|---|---|
| CAS | 1-naphthalenol |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 2542190 | Garcia-Caballero T, Morel G: Immunocytochemical localization of ACTH-like immunoreactivity in rat glomerulosa and fasciculata cells. Histochemistry. 1989;91(3):205-12. The antigen-antibody reaction was detected by PAP complexes (revealed by 4-chloro-1-naphthol) or with protein A-colloidal gold or IgG-colloidal gold. |
81(1,1,1,1) | Details |
| 2423145 | Ishiguro T, Sakaguchi H, Fukui M, Sugitachi I: Serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis. Biol Res Pregnancy Perinatol. 1985;6(3):114-7. The method involves the first dimension electrophoresis in agarose gel containing concanavalin A (Con A) or lentil lectin (LCH), the second dimension immunoelectrophoresis in agarose gel containing polyclonal antibody against AFP, reaction with peroxidase-conjugated Protein A and staining with 4-methoxy-1-naphthol. |
31(0,1,1,1) | Details |
| 6607959 | Beyer CF: A 'dot-immunobinding assay' on nitrocellulose membrane for the determination of the immunoglobulin class of mouse monoclonal antibodies. J Immunol Methods. 1984 Feb 24;67(1):79-87. The strips are then incubated with protein A conjugated to peroxidase, and finally with the peroxidase substrate 4-chloro-1-naphthol. |
81(1,1,1,1) | Details |
| 11437353 | Soars MG, Smith DJ, Riley RJ, Burchell B: Cloning and characterization of a canine UDP-glucuronosyltransferase. Arch Biochem Biophys. 2001 Jul 15;391(2):218-24. The enzyme expressed stably in V79 cells predominantly catalyzed the glucuronidation of simple, planar phenols (e.g., for 1-naphthol, K (m) = 41 microM, V (max) = 0.07 nmol/min/mg protein), a class of compounds extensively glucuronidated by human UGT1A6. |
31(0,1,1,1) | Details |
| 16347258 | Fletcher J: Filter Paper Dot-Immunobinding Assay for Detection of Spiroplasma citri. Appl Environ Microbiol. 1987 Jan;53(1):183-184. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus peroxide. |
81(1,1,1,1) | Details |
| 16439107 | Wu Z, Wu J, Wang S, Shen G, Yu R: An amplified mass piezoelectric immunosensor for Schistosoma japonicum. . Biosens Bioelectron. 2006 Aug 15;22(2):207-12. Epub 2006 Jan 24. And the horseradish peroxidase labeled protein A conjugate which was bounded to the SjAb by a "sandwich" format was used as a biocatalyst for the oxidative precipitation of 4-chloro-1-naphthol by H (2) O (2) to yield the insoluble product benzo-4-chlorohexadienone, resulting in an amplified mass sensing of antigen-antibody interaction. |
31(0,1,1,1) | Details |
| 6625179 | Esen A, Conroy JM, Wang SZ: A simple and rapid dot-immunobinding assay for zein and other prolamins. Anal Biochem. 1983 Jul 15;132(2):462-7. -solubilized zein is spotted onto chromatography paper disks or strips, incubated with antiserum (30-60 min), washed with several changes of Tris-buffered saline (10 min), incubated with protein A-peroxidase (30 min), and washed with several changes of Tris-buffered saline (10 min). the binding of zein-specific antibody is visualized by peroxidase-catalyzed color production from 4-chloro-1-naphthol. |
31(0,1,1,1) | Details |
| 7989432 | Wagih E, Melouk H, Sherwood J: Mirror image in vivo electroblotting technique, a new technique for visualizing virus particles electrophoretically transferred from infected leaves to nitrocellulose membranes. J Virol Methods. 1994 Jul;48(2-3):145-53. The virus-bound antibodies were located by protein A-peroxidase or protein A-alkaline phosphatase conjugate followed by the peroxidase substrate mixture, 4-chloro-1-naphthol and H2O2, in the first case or the alkaline phosphatase substrate mixture, 5-bromo-4-chloro-3-indolyl and Nitroblue tetrazolium in the second case. |
31(0,1,1,1) | Details |
| 11286340 | Penalva J, Puchades R, Maquieira A, Gee S, Hammock BD: Development of immunosensors for the analysis of 1-naphthol in organic media. Biosens Bioelectron. 2000 Jun;15(3-4):99-106. Immunoreagents were immobilized on controlled pore glass (CPG), hidroxysuccinimide agarose gel or on azlactone Protein A/G supports. |
3(0,0,0,3) | Details |
| 6428319 | Mackenzie PI, Hjelmeland LM, Owens IS: Purification and immunochemical characterization of a low-pI form of UDP glucuronosyltransferase from mouse liver. Arch Biochem Biophys. 1984 Jun;231(2):487-97. All activities, however, were immunoadsorbed in the presence of protein A-Sepharose. This enzyme had a broad substrate specificity and was mainly responsible for the microsomal capacity to glucuronidate 1-naphthol, and morphine. |
2(0,0,0,2) | Details |
| 10489531 | Penalva J, Puchades R, Maquieira A: Analytical properties of immunosensors working in organic media. Anal Chem. 1999 Sep 1;71(17):3862-72. On the basis on heterogeneous competitive enzyme formats, three assay types were used: immobilized antibodies (direct format), immobilized hapten conjugates (indirect format), and capture format based on immobilized protein A/G. Monoclonal and polyclonal antibodies for carbaryl and 1-naphthol were used to develop sensors in different organic solvent mixtures. |
1(0,0,0,1) | Details |
| 2885949 | Gandhi BM: A dot immunobinding assay (DIA) on nitrocellulose membrane for the serological detection of antibodies to Entamoeba histolytica. Trans R Soc Trop Med Hyg. 1986;80(6):883-5. The strips were incubated with test samples and later with horseradish peroxidase (HRPO) labelled protein-A conjugate. A dark blue spot was obtained by treatment with peroxidase substrate, 4-chloro-1-naphthol, in positive samples. |
1(0,0,0,1) | Details |
| 10798290 | Teng SF, Sproule K, Husain A, Lowe CR: Affinity chromatography on immobilized "biomimetic" ligands. J Chromatogr B Biomed Sci Appl. 2000 Mar 31;740(1):1-15. A synthetic bifunctional ligand (22/8) comprising a triazine scaffold substituted with 3- (22) and 4-amino-1-naphthol (8) has been designed, synthesised, characterised and immobilized on agarose beads to create a robust, highly selective affinity adsorbent for human immunoglobulin G (IgG). The adsorbent shows similar selectivity to immobilized protein A and binds IgG from a number of species. |
1(0,0,0,1) | Details |
| 8161205 | Ouzzine M, Pillot T, Fournel-Gigleux S, Magdalou J, Burchell B, Siest G: Expression and role of the human liver UDP-glucuronosyltransferase UGT1*6 analyzed by specific antibodies raised against a hybrid protein produced in Escherichia coli. Arch Biochem Biophys. 1994 Apr;310(1):196-204. The novel approach consists of the expression of a N-terminal UGT polypeptide fused to Staphylococcus aureus protein A in Escherichia coli and a single step purification of the fusion protein by immunoaffinity chromatography. In human liver microsomes, specific immunoinhibition analysis demonstrated that glucuronidation by UGT1*6 represented 20 to 50% of the total 1-naphthol UGT activity with a good correlation with the amount of protein selectively quantified on immunoblot. |
1(0,0,0,1) | Details |
| 2865313 | Kumar S, Band AH, Samantaray JC, Dang N, Talwar GP: A dot enzyme-linked immunosorbent assay for detection of antibodies against Entamoeba histolytica. J Immunol Methods. 1985 Oct 24;83(1):125-33. The antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (BSA) and incubated with 3-fold dilutions of patient sera followed by incubation with protein A conjugated to peroxidase. Enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. |
1(0,0,0,1) | Details |