Protein Information

Name F12
Synonyms Coagulation factor XII; Coagulation factor XII precursor; Coagulation factor XII Mie; F12; F12 protein; Factor XII; HAF; Hageman factor…

Compound Information

Name streptomycin
CAS

Reference List

PubMed Abstract RScore(About this table)
16974093 Reuther T, Kettmann C, Scheer M, Kochel M, Iida S, Kubler AC: Cryopreservation of osteoblast-like cells: viability and differentiation with replacement of fetal bovine serum in vitro. Cells Tissues Organs. 2006;183(1):32-40.

In the cell culture of cryopreserved and fresh osteoblast-like cells, we substituted Dulbecco's modification of Eagle's medium (DMEM)/Ham's F12 plus 1% penicillin/streptomycin with autologous serum, human serum albumin and Biseko for fetal bovine serum.
43(0,1,3,3) Details
17456355 Fang JS, Deng YW, Li MC, Chen FH, Wang YJ, Lu M, Fang F, Wu J, Yang ZY, Zhou XY, Wang F, Chen C: [Isolation and identification of brain tumor stem cells from human brain neuroepithelial tumors]. Zhonghua Yi Xue Za Zhi. 2007 Jan 30;87(5):298-303.

The tumor cells were seeded at a concentration of 200,000 viable cells per mL into serum-free DMEM-F12 medium simply supplemented with B27, human basic fibroblast growth factor (20 microg/L), human epidermal growth factor (20 microg /L), insulin (4 U/L), L-glutamine, penicillin and streptomycin.
8(0,0,1,3) Details
19271684 Bruckova L, Soukup T, Moos J, Moosova M, Pavelkova J, Rezabek K, Visek B, Mokry J: The cultivation of human granulosa cells. Acta Medica (Hradec Kralove). 2008;51(3):165-72.

GCs expansion medium consisted of DMEM/F12, 2% FCS, ascorbic acid, dexamethasone, L-glutamine, gentamycine, penicillin, streptomycin and growth factors (EGF, bFGF).
7(0,0,1,2) Details
17284244 Brown NL, Sandy JR: Tails of the unexpected: palatal medial edge epithelium is no more specialized than other embryonic epithelium. Orthod Craniofac Res. 2007 Feb;10(1):22-35.

METHODS: Organs were cultured in 2 ml of DMEM/F12 supplemented with 300 microg/ml L-glutamine and 1% penicillin/streptomycin.
6(0,0,1,1) Details
16383034 Tao K, Chen B, Xie ST: [In vitro isolation, cultivation and identification of sebocytes and eccrine sweat gland cells from human fetal skin]. Zhonghua Shao Shang Za Zhi. 2005 Oct;21(5):343-6.

DMEM/F12 (1: 1) was used as the basic culture medium, supplemented with fetal bovine serum, recombinant human epidermal growth factor, L-glutamine, Hydrocortisone, choleratoxin, penicillin and streptomycin as the medium for sebocytes, or fetal bovine serum, recombinant human epidermal growth factor, triiodothyronine, hydrocortisone, insulin, transferrin, sodium selenite to the medium for eccrine sweat gland duct cells.
6(0,0,1,1) Details
15175915 Yeung CK, Chan KP, Chan CK, Pang CP, Lam DS: Cytotoxicity of triamcinolone on cultured human retinal pigment epithelial cells: comparison with dexamethasone and hydrocortisone. Jpn J Ophthalmol. 2004 May-Jun;48(3):236-42.


METHODS: The ARPE19 cell line was cultured and maintained in a 1 : 1 mixture of Dulbecco's modified Eagle's medium and HAMS F12 medium containing 3 mM l-glutamine supplemented with 10% fetal bovine serum, penicillin G, and streptomycin sulfate.
1(0,0,0,1) Details
16531078 Shimizu K, Sakai M, Ando M, Chiji H, Kawada T, Mineo H, Taira T: Newly developed primary culture of rat visceral adipocytes and their in vitro characteristics. Cell Biol Int. 2006 Apr;30(4):381-8. Epub 2006 Mar 10.


Modified Dulbecco's modified Eagle medium (DMEM)/F12 containing 17 microM pantothenic acid, 33 microM biotin, 100 microM ascorbic acid, 1 microM octanoic acid, 50 nM triiodothyronine, 10 microg/ml insulin, 10% newborn calf serum (NCS), 100 units/ml penicillin and 100 microg/ml streptomycin was used as a basal culture medium, which did not contain any synthetic compounds usually used to promote adipogenesis, such as indomethacin, dexamethasone, or peroxisome proliferator-activated receptor (PPAR)-gamma agonists.
0(0,0,0,0) Details