Protein Information

Name EF Tu
Synonyms CCS 3; CCS3; CTCL tumor antigen; EEF 1; EEF1; EEF1A; EEF1A1; EF 1 alpha 1…

Compound Information

Name streptomycin
CAS

Reference List

PubMed Abstract RScore(About this table)
16843583 Ninio J: Multiple stages in codon-anticodon recognition: double-trigger mechanisms and geometric constraints. Biochimie. 2006 Aug;88(8):963-92. Epub 2006 Jun 27.

Streptomycin and most EF-Tu mutants as well as high accuracy ribosomal mutants would produce specific alterations at stage 2, which are mapped on the stage 2 kinetic mechanism.
32(0,1,1,2) Details
19095621 Gregory ST, Carr JF, Dahlberg AE: A signal relay between ribosomal protein S12 and elongation factor EF-Tu during decoding of mRNA. RNA. 2009 Feb;15(2):208-14. Epub 2008 Dec 17.

The streptomycin-resistance phenotypes of H76R, R37C, and K53E are all abolished by the mutation A375T in EF-Tu.
12(0,0,1,7) Details
15004548 Gromadski KB, Rodnina MV: Streptomycin interferes with conformational coupling between codon recognition and GTPase activation on the ribosome. Nat Struct Mol Biol. 2004 Apr;11(4):316-22. Epub 2004 Mar 7.

Streptomycin altered the rates of GTP hydrolysis by elongation factor Tu (EF-Tu) on cognate and near-cognate codons, resulting in almost identical rates of GTP hydrolysis and virtually complete loss of selectivity.
6(0,0,1,1) Details