Protein Information

Name NDH2
Synonyms ATP dependent RNA helicase A; DDX 9; DDX9; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 (RNA helicase A); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 RNA helicase A; DEAH (Asp Glu Ala His) box polypeptide 9; DEAH box protein 9; DHX 9…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
19097788 Dong CK, Patel V, Yang JC, Dvorin JD, Duraisingh MT, Clardy J, Wirth DF: Type II NADH dehydrogenase of the respiratory chain of Plasmodium falciparum and its inhibitors. Bioorg Med Chem Lett. 2009 Feb 1;19(3):972-5. Epub 2008 Nov 24.

Plasmodium falciparum NDH2 (pfNDH2) is a non-proton pumping, rotenone-insensitive alternative enzyme to the multi-subunit NADH:ubiquinone oxidoreductases (Complex I) of many other eukaryotes.
6(0,0,1,1) Details
8804429 Finel M: Genetic inactivation of the H (+)-translocating NADH:ubiquinone oxidoreductase of Paracoccus denitrificans is facilitated by insertion of the ndh gene from Escherichia coli. FEBS Lett. 1996 Sep 9;393(1):81-5.

This was achieved by constructing a deletion plasmid in which most of the coding regions of nqo8 and nqo9 were replaced by the ndh gene of Escherichia coli that encodes an alternative NADH:ubiquinone oxidoreductase (NDH2), and a kanamycin resistance gene.
Subsequent homologous recombination gave rise to a mutant the membranes of which catalyzed rotenone-insensitive NADH oxidation, but which did not oxidize deamino-NADH.
2(0,0,0,2) Details