Protein Information

Name pyridine nucleotide transhydrogenase
Synonyms NNT; NNT protein; Nicotinamide nucleotide transhydrogenase; Pyridine nucleotide transhydrogenase; NNT proteins; Nicotinamide nucleotide transhydrogenases; Pyridine nucleotide transhydrogenases

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
1403417 Fioravanti CF, McKelvey JR, Reisig JM: Energy-linked mitochondrial pyridine nucleotide transhydrogenase of adult Hymenolepis diminuta. J Parasitol. 1992 Oct;78(5):774-8.

The isolated particles displayed rotenone-sensitive NADH utilization and the reversible transhydrogenase, with the NADPH----NAD+ transhydrogenation being more prominent.
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4395037 Rydstrom J, da Cruz AT, Ernster L: Factors governing the kinetics and steady state of the mitochondrial nicotinamide nucleotide transhydrogenase system. Eur J Biochem. 1970 Nov;17(1):56-62.

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549647 Klimek J, Boguslawski W, Zelewski L: The relationship between energy generation and cholesterol side-chain cleavage reaction in the mitochondria from human term placenta. Biochim Biophys Acta. 1979 Oct 18;587(3):362-72.

ADP and ATP stimulated the malate, succinate and alpha-ketoglutarate-supported progesterone biosynthesis probably via the energy-dependent pyridine nucleotide transhydrogenase activation.
The effect of ADP was abolished by rotenone and antimycin in the presence of malate or alpha-ketoglutarate. 3.
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1757194 Goyal N, Gupta S, Katiyar JC, Srivastava VM: NADH oxidase and fumarate reductase of Ancylostoma ceylanicum. Int J Parasitol. 1991 Oct;21(6):673-6.

The membranes also exhibited the presence of a reduced pyridine nucleotide transhydrogenase activity which transferred hydrogen from NADPH to NAD.
Amongst respiratory inhibitors, rotenone (Site I inhibitor) markedly depressed both NADH oxidase and fumarate reductase while others, namely antimycin-A, KCN and azide, had a lesser effect.
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2267725 Goyal N, Srivastava VM: Mitochondrial NADH oxidase activity of Setaria cervi. Vet Parasitol. 1990 Nov;37(3-4):229-36.

The membrane preparation also exhibited the presence of a reduced pyridine nucleotide transhydrogenase which converted NADPH into NADH by transferring a hydride ion.
The oxidase activity was inhibited by all the respiratory inhibitors examined, with the greatest sensitivity to rotenone, a site I-specific inhibitor.
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