| Name | pyruvate dehydrogenase (protein family or complex) |
|---|---|
| Synonyms | Pyruvate dehydrogenase; Pyruvate dehydrogenases |
| Name | rotenone |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 4404963 | Kather H, Rivera M, Brand K: Interrelationship and control of metabolism and lipogenesis in isolated fat-cells. Biochem J. 1972 Aug;128(5):1097-102. Rotenone, which is known to inhibit electron transport in the respiratory chain, blocked breakdown at the site of pyruvate dehydrogenase. |
81(1,1,1,1) | Details |
| 444478 | Lichtor T, Tung B, Getz GS: Cytoplasmically inherited respiratory deficiency of a mouse fibroblast line which is resistant to rutamycin. Biochemistry. 1979 Jun 12;18(12):2582-90. Levels of rotenone-sensitive -cytochrome c reductase and decarboxylase of the pyruvate dehydrogenase complex are markedly depressed in the mutant cells. |
31(0,1,1,1) | Details |
| 176149 | Batenburg JJ, Olson MS: Regulation of pyruvate dehydrogenase by fatty acid in isolated rat liver mitochondria. J Biol Chem. 1976 Mar 10;251(5):1364-70. Addition of fatty acid caused an inactivation of pyruvate dehydrogenase in mitochondria incubated under State 3 conditions plus hexokinase), in uncoupled, oligomycin-treated mitochondria, and in rotenone--treated mitochondria, but not in uncoupled mitochondria or in mitochondria incubated under State 4 conditions. |
10(0,0,0,10) | Details |
| 123530 | Chiang PK, Sacktor B: Control of pyruvate dehydrogenase activity in intact cardiac mitochondria. J Biol Chem. 1975 May 10;250(9):3399-408. in the presence of rotenone, strongly inhibited inactivation. |
9(0,0,0,9) | Details |
| 196589 | Kerbey AL, Radcliffe PM, Randle PJ: Diabetes and the control of pyruvate dehydrogenase in rat heart mitochondria by concentration ratios of / of reduced/oxidized and of / Biochem J. 1977 Jun 15;164(3):509-19. This was based on comparison between mitochondria incubated with 0.4mM- or 1mM- and mitochondria incubated with 0.4mM-/-rotenone. 3. |
6(0,0,0,6) | Details |
| 19747108 | Torrentino-Madamet M, Desplans J, Travaille C, James Y, Parzy D: Microaerophilic Respiratory Metabolism of Plasmodium falciparum Mitochondrion as a Drug Target. Curr Mol Med. 2009 Sep 13. In addition, we provide details on certain characteristics like the lack of pyruvate dehydrogenase, the existence of a rotenone-insensitive NADH-dehydrogenase, the possible existence of an alternative oxidase, and uncoupled proteins. |
6(0,0,1,1) | Details |
| 20205678 | Torrentino-Madamet M, Desplans J, Travaille C, James Y, Parzy D: Microaerophilic respiratory metabolism of Plasmodium falciparum mitochondrion as a drug target. Curr Mol Med. 2010 Feb 1;10(1):29-46. In addition, we provide details on certain characteristics like the lack of pyruvate dehydrogenase, the existence of a rotenone-insensitive NADH-dehydrogenase, the possible existence of an alternative oxidase, and uncoupled proteins. |
6(0,0,1,1) | Details |
| 184082 | Hansford RG: Studies on the effects of -SH: and ratios on the interconversion of active and inactive pyruvate dehydrogenase in isolated rat heart mitochondria. J Biol Chem. 1976 Sep 25;251(18):5483-9. The content of -SH and of suspensions of rat heart mitochondria was stabilized by the addition of and acetyl- in the presence of the respiratory inhibitor rotenone. |
4(0,0,0,4) | Details |
| 1116996 | Taylor SI, Mukherjee C, Jungas RL: Regulation of pyruvate dehydrogenase in isolated rat liver mitochondria. J Biol Chem. 1975 Mar 25;250(6):2028-35. When mitochondria were incubated in State 4 with and rotenone, the addition of increased PDHa activity more than 10-fold without appreciably altering the mitochondrial ATP:ADP ratio. |
4(0,0,0,4) | Details |
| 2464995 | Moreno-Sanchez R, Hansford RG: Dependence of cardiac mitochondrial pyruvate dehydrogenase activity on intramitochondrial free Ca2+ concentration. Biochem J. 1988 Dec 1;256(2):403-12. The value of the Kd of indo-1-Ca2+ in the mitochondrial matrix was determined to be 95 nM, on the basis of equilibration of [Ca2+] m with the extramitochondrial free Ca2+ ([Ca2+] o) in the presence of rotenone, nigericin, valinomycin and Br-A23187. (2) [Ca2+] m responded to energization/de-energization protocols, the inhibition of Ca2+-uptake by Ruthenium Red and the potentiation of Ca2+-efflux by Na+ in a manner which was consistent with the known kinetic properties of the mitochondrial Ca2+-transport processes. (3) The concentration gradient [Ca2+] m/[Ca2+] o was found to be near unity (0.82 +/- 0.18) when mitochondria were incubated in media containing 10 mM-Na+; the additional presence of 1 mM-Mg2+ reduced the gradient to values below unity (0.26 +/- 0.03). |
3(0,0,0,3) | Details |
| 8569751 | Carter TC, Coore HG: Effects of on pyruvate dehydrogenase kinase of rat heart. . Mol Cell Biochem. 1995 Aug-Sep;149-150:71-5. Similar behaviour was observed in mPDC from fed and starved animals when rotenone was present, at 1 mM concentration stimulated PDHK from hearts of fed animals but was without effect at 10 mM. |
2(0,0,0,2) | Details |
| 17254815 | Vellucci VF, Gygax SE, Hostetter MK: Involvement of Candida albicans pyruvate dehydrogenase complex protein X (Pdx1) in filamentation. Fungal Genet Biol. 2007 Oct;44(10):979-90. Epub 2006 Dec 16. |
2(0,0,0,2) | Details |
| 8417013 | Zaidan E, Sims NR: Selective reductions in the activity of the pyruvate dehydrogenase complex in mitochondria isolated from brain subregions following forebrain ischemia in rats. J Cereb Blood Flow Metab. 1993 Jan;13(1):98-104. No significant changes were observed in the activity of two other mitochondrial markers, rotenone-sensitive -cytochrome c oxidoreductase and alpha-ketoglutarate dehydrogenase. |
2(0,0,0,2) | Details |
| 19526285 | Mallajosyula JK, Chinta SJ, Rajagopalan S, Nicholls DG, Andersen JK: Metabolic control analysis in a cellular model of elevated MAO-B: relevance to Parkinson's disease. Neurotox Res. 2009 Oct;16(3):186-93. Epub 2009 Mar 5. In addition to KGDH, we assessed the activities and substrate-mediated respiration of complex I, pyruvate dehydrogenase (PDH), succinate dehydrogenase (SDH), and mitochondrial aconitase in the absence and presence of complex-specific inhibitors in specific and mixed substrate conditions in mitochondria from our MAO-B elevated cells versus controls. |
1(0,0,0,1) | Details |
| 10844979 | Obungu VH, Kiaira JK, Olembo NK, Njogu MR: Pathways of catabolism in procyclic Trypanosoma congolense. Indian J Biochem Biophys. 1999 Oct;36(5):305-11. Studies of respiration on in procyclic Trypanosoma congolense in the presence of rotenone, antimycin, salicylhydroxamic acid and have indicated the presence of NADH dehydrogenase, cytochrome b-c1, cytochrome aa3, trypanosome alternate oxidase and reductase/succinate dehydrogenase pathway that contributes electrons to of the respiratory chain. Phosphoenolpyruvate carboxykinase, pyruvate dehydrogenase, succinate dehydrogenase, (+)-linked malic enzyme, reductase, malate dehydrogenase, and alpha-ketoglutarate dehydrogenase and glycerol kinase on the other hand had specific activities greater than 60 nanomoles/min/mg protein. |
1(0,0,0,1) | Details |
| 3009544 | Robinson BH, Ward J, Goodyer P, Baudet A: Respiratory chain defects in the mitochondria of cultured skin fibroblasts from three patients with lacticacidemia. J Clin Invest. 1986 May;77(5):1422-7. The cultured skin fibroblasts from three patients with lacticacidemia were found to have low rates of 1-[14C] oxidation in the face of normal pyruvate dehydrogenase activity. Measurement of Rotenone-sensitive -cytochrome c reductase in mitochondrial preparations from skin fibroblasts showed that two of the patients had 14 and 18%, respectively, of control activity. |
1(0,0,0,1) | Details |
| 14607783 | Peterside IE, Selak MA, Simmons RA: Impaired oxidative phosphorylation in hepatic mitochondria in growth-retarded rats. Am J Physiol Endocrinol Metab. 2003 Dec;285(6):E1258-66. Increased levels of manganese superoxide dismutase suggest that an antioxidant response has been mounted, and hydroxynonenal (HNE) modification of pyruvate dehydrogenase E2-(catalytic) and E3-binding protein subunits suggests that HNE-induced inactivation of this key enzyme may play a role in the mechanism of injury. Rotenone-sensitive -O2 oxidoreductase activity was similar in control and IUGR mitochondria at all ages, showing that the defect responsible for decreased and alpha-ketoglutarate oxidation in IUGR liver precedes the electron transport chain and involves and alpha-ketoglutarate dehydrogenases. |
1(0,0,0,1) | Details |
| 8786242 | Xu DP, Wells WW: dependent regeneration of from in rat liver mitochondria. J Bioenerg Biomembr. 1996 Feb;28(1):77-85. Electron transport inhibitors, rotenone and antimycin A, further enhanced the reduction. Pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase reduced to in an and or alpha-ketoglutarate dependent fashion. |
1(0,0,0,1) | Details |
| 16667685 | Pascal N, Dumas R, Douce R: Comparison of the Kinetic Behavior toward Nucleotides of -Linked Dehydrogenases from Plant Mitochondria. Plant Physiol. 1990 Sep;94(1):189-193. In this article we compare the kinetic behavior toward nucleotides (+), of (+)-malic enzyme, pyruvate dehydrogenase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, and glycine decarboxylase extracted from pea (Pisum sativum) leaf and potato (Solanum tuberosum) tuber mitochondria. It is suggested that (+)-linked malic enzyme, when fully activated, is able to raise the matricial level up to the required concentration to fully engage the rotenone-resistant internal NADH-dehydrogenase, whose affinity for is weaker than complex I. |
1(0,0,0,1) | Details |
| 19763263 | Johnson F, Kaplitt MG: Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders. PLoS One. 2009 Sep 18;4(9):e7100. Additionally our gene expression studies, using rotenone (an inhibitor of Complex I) showed Omi expression was silenced when pdhb and idh3a were increased when a sub-lethal dose was applied. Pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH) are two key Kreb's cycle enzymes that catalyse oxidative decarboxylation control points in mitochondrial respiration. |
1(0,0,0,1) | Details |
| 15356189 | Starkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF: Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive species. J Neurosci. 2004 Sep 8;24(36):7779-88. In the absence of ADP or in the presence of rotenone, H (2) O (2) production rates correlated with the reduction level of mitochondrial with various substrates, with the exception of alpha-ketoglutarate. Isolated mitochondrial alpha-ketoglutarate dehydrogenase (KGDHC) and pyruvate dehydrogenase (PDHC) complexes produced and H (2) O (2). |
1(0,0,0,1) | Details |
| 1456730 | Ofenstein JP, Dandurand DM, Kiechle FL: Assessment of mitochondrial function in cells grown in tissue culture. Ann Clin Lab Sci. 1992 Nov-Dec;22(6):406-13. To assess mitochondrial function (pyruvate dehydrogenase [PDH] activity), cells were grown in the appropriate media to confluence, rinsed and incubated in free media containing 25 microM L-lactate and [1-14C]-D,L-lactate. The mitochondrial inhibitor rotenone (25 microM) decreased assay activity by > 75 percent in CHO and BC3H-1 cells. |
1(0,0,0,1) | Details |
| 4854074 | Wieland OH, Portenhauser R: Regulation of pyruvate-dehydrogenase interconversion in rat-liver mitochondria as related to the phosphorylation state of intramitochondrial nucleotides. Eur J Biochem. 1974 Jun 15;45(2):577-88. |
1(0,0,0,1) | Details |
| 7082719 | Mita M, Hall PF: Metabolism of round spermatids from rats: lactate as the preferred substrate. Biol Reprod. 1982 Apr;26(3):445-55. Synthesis of ATP with lactate and with is inhibited by rotenone, rutamycin or 2,4-dinitrophenol. |
0(0,0,0,0) | Details |