Protein Information

Name acyl CoA dehydrogenases
Synonyms short chain acyl coenzyme A dehydrogenase; ACAD 3; ACAD3; ACADS; Acyl CoA dehydrogenase; Acyl Coenzyme A dehydrogenase; Butyryl CoA dehydrogenase; Butyryl Coenzyme A dehydrogenase…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
1668635 Kunz WS: Application of the theory of steady-state flux control to mitochondrial beta-oxidation. Biomed Biochim Acta. 1991;50(12):1143-57.

The theory of steady-state flux control was applied to characterize the regulation of beta-oxidation flux in uncoupled rat liver mitochondria oxidizing palmitoylcarnitine in the presence of rotenone, malonate and the beta-hydroxybutyrate/acetoacetate redox buffer.
It was found that at low flux rates the flux control was distributed mainly between acyl-CoA dehydrogenase and beta-hydroxyacyl-CoA dehydrogenase (Ci = 0.89).
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648633 Osmudsen H: Effects of pent-4-enoate on flux through acyl-CoA dehydrogenases of beta-oxidation in intact rat liver mitochondria. FEBS Lett. 1978 Apr 15;88(2):219-22.

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6704106 Komuniecki R, Fekete S, Thissen J: 2-Methylbutyryl CoA dehydrogenase from mitochondria of Ascaris suum and its relationship to NADH-dependent 2-methylcrotonyl CoA reduction. Biochem Biophys Res Commun. 1984 Feb 14;118(3):783-8.

Acyl CoA dehydrogenase and electron-transfer flavoprotein have been isolated and partially purified from mitochondria of the anaerobic nematode, Ascaris suum.
In fact, incubations of A. suum mitochondrial membranes with electron-transfer flavoprotein, 2-methylbutyryl CoA dehydrogenase, 2-methylcrotonyl CoA and NADH resulted in a substantial, rotenone-sensitive, 2-methylbutyrate synthesis.
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12517982 Blackstone NW: Redox signaling in the growth and development of colonial hydroids. J Exp Biol. 2003 Feb;206(Pt 4):651-8.

Acyl CoA dehydrogenase, which catalyzes the first step in the mitochondrial beta-oxidation of fatty acids, carries electrons to coenzyme Q, thus bypassing complex I.
Oxygen uptake of colonies was measured to determine comparable physiological doses of antimycin A (1) (an inhibitor of complex III), rotenone (an inhibitor of complex I) and carbonyl cyanide m-chlorophenylhydrazone (CCCP; an uncoupler of oxidative phosphorylation).
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883956 Osmundsen H, Bremer J: A spectrophotometric procedure for rapid and sensitive measurements of beta-oxidation. Biochem J. 1977 Jun 15;164(3):621-33.

The procedure relies on the ability of ferricyanide to trap reducing equivalents generated by the acyl-CoA dehydrogenases (EC 1.3.99.3).
Oxaloacetate or acetoacetate was used to stimulate the rate of beta-oxidation by rotenone-blocked mitochondria.
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