| 18402771 |
Lee J, Huang MS, Yang IC, Lai TC, Wang JL, Pang VF, Hsiao M, Kuo MY: Essential roles of caspases and their upstream regulators in rotenone-induced apoptosis. Biochem Biophys Res Commun. 2008 Jun 20;371(1):33-8. Epub 2008 Apr 8.
In the present study, we examined whether caspases and their upstream regulators are involved in rotenone-induced cytotoxicity. Rotenone significantly inhibited the proliferation of oral cancer cell lines in a dose-dependent manner compared to normal oral mucosal fibroblasts. Flow cytometric analysis of DNA content showed that rotenone treatment induced apoptosis following G2/M arrest. Western blotting showed activation of both the caspase-8 and caspase-9 pathways, which differed from previous studies conducted in other cell types. Furthermore, p53 protein and its downstream pro-apoptotic target, Bax, were induced in SAS cells after treatment with rotenone. Rotenone-induced apoptosis was inhibited by antioxidants (glutathione, N-acetylcysteine, and tiron). In conclusion, our results demonstrate significant involvement of caspases and their upstream regulators in rotenone-induced cytotoxicity. |
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