| ID | 25 |
|---|---|
| Name | phospholipase C |
| Synonyms | PLC L; PLCE; PLC L (PLC epsilon); PLCL; PLCL 1; PLCL1; PLDL 1; PLDL1… |
| ID | 332 |
|---|---|
| Name | 4-aminopyridine |
| CAS | 4-pyridinamine |
| PubMed | Abstract | RScore(About this table) |
|---|---|---|
| 12065624 | Donald AN, Wallace DJ, McKenzie S, Marley PD: Phospholipase C-mediated signalling is not required for -induced catecholamine secretion from bovine chromaffin cells. J Neurochem. 2002 Jun;81(5):1116-29. A possible role for signalling through phospholipase C in -induced catecholamine secretion from bovine chromaffin cells has been investigated. Secretion evoked by over 10 min was not prevented by inhibiting receptors with 2-APB, by blocking ryanodine receptors with a combination of ryanodine and or by depleting intracellular Ca (2+) stores by pretreatment with thapsigargin. Inhibition of protein kinase C with Ro31-8220 also failed to reduce secretion. Inhibition of phospholipase C with ET-18-OCH (3) reduced both - and K (+) -induced responses by 70-80% without reducing their secretory responses. Stimulating phospholipase C with Pasteurella multocida toxin did not evoke secretion or enhance the secretory response to The secretory response to was little affected by tetrodotoxin or by substituting extracellular Na (+) with N -methyl-d-glucamine (+) or (+), or by substituting external Cl (-) with (-). Blocking various K (+) channels with apamin, charybdotoxin, Ba (2+), tetraethylammonium, 4-aminopyridine, tertiapin or glibenclamide failed to reduce the ability of to evoke secretion. These results indicate that evokes secretion by a mechanism that does not require -mediated mobilization of stored Ca (2+), diacylglycerol-mediated activation of protein kinase C, or activation of phospholipase C. The results are consistent with acting by depolarizing chromaffin cells through a phospholipase C-independent mechanism. |
3(0,0,0,3) |