Protein Information

ID 2969
Name PSST
Synonyms CI 20kD; Complex I 20kD; My017 protein; NADH dehydrogenase (ubiquinone) Fe S protein 7 (20kD) (NADH coenzyme Q reductase); NADH ubiquinone oxidoreductase Fe S protein 7; NADH ubiquinone oxidoreductase 20 kDa subunit; NADH ubiquinone oxidoreductase Fe S protein 7 variant; NDUFS 7…

Compound Information

ID 1341
Name rotenone
CAS

Reference

PubMed Abstract RScore(About this table)
7957254 Finel M, Majander AS, Tyynela J, De Jong AM, Albracht SP, Wikstrom M: Isolation and characterisation of subcomplexes of the mitochondrial NADH:ubiquinone oxidoreductase (complex I). Eur J Biochem. 1994 Nov 15;226(1):237-42.
Enzymically active subcomplexes were purified from bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I) by sucrose-gradient centrifugation in the presence of detergents. These subcomplexes, named I lambda, IS, and I lambda S, catalyse ferricyanide and ubiquinone-1 (Q-1) reduction by NADH at similar rates to complex I, but do not catalyse the reduction of decylubiquinone. In addition, the Q-1 reductase activity of all the subcomplexes is insensitive to rotenone. Chemical and EPR analyses of the subcomplexes show that FMN and all the Fe-S clusters of complex I are present, but that the line shape of cluster 2 is modified. The smallest subcomplex, I lambda S, contains only approximately 13 subunits, as compared to approximately 22 in the previously described subcomplex I alpha [Finel, M., Skehel, J. M., Albracht, S. J. P., Fearnley, I. M. & Walker, J. E. (1992) Biochemistry 31, 11425-11434], but it retains the 75-, 51-, 49-, 30-, 24-, 23- (TYKY) and 20-kDa (PSST) subunits, which are suggested to form a functional core that comprises the EPR-detectable Fe-S clusters 1-4, and FMN. The structural and functional implications of such an arrangement are discussed.
1(0,0,0,1)