| 12628584 |
Wu A, Li L, Liu Y: Deltamethrin induces apoptotic cell death in cultured cerebral cortical neurons. Toxicol Appl Pharmacol. 2003 Feb 15;187(1):50-7.
In this study we investigated the induction of apoptotic cell death and its potential mechanisms in cultured cortical neurons in response to deltamethrin exposure. The cultured cortical neurons were treated at 7 days with deltamethrin at concentrations of 10, 100, and 1000 nM, respectively. MTT assay showed that higher concentrations of deltamethrin (100 and 1000 nM) decreased neuronal viability in a time- and dose-dependent way. TUNEL staining revealed that numerous apoptotic cells appeared in the treated cultures compared to controls at 24, 48, and 72 h after treatment of 100 nM deltamethrin. Western blot analysis demonstrated that p53 and Bax expression were dramatically increased at the same time points, whereas Bcl-2 expression was significantly reduced at all time points after deltamethrin treatment. Further, we found that nitric oxide synthase inhibitor N (G)-nitro-L-arginine prevented deltamethrin-induced neuronal apoptosis and altered expression of p53, Bax, and Bcl-2. These results suggest that nitric oxide synthase might mediate deltamethrin-elicited neuronal apoptosis through modulating the expression of apoptosis-related genes. |
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