| Name | ATPase |
|---|---|
| Synonyms | ATP7A; MK; ATPase; Cation transporting ATPase; ATP7A protein; ATPase Cu(2+) transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1… |
| Name | rotenone |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 2862915 | Herweijer MA, Berden JA, Kemp A, Slater EC: Inhibition of energy-transducing reactions by 8-nitreno-ATP covalently bound to bovine heart submitochondrial particles: direct interaction between ATPase and redox enzymes. Biochim Biophys Acta. 1985 Aug 28;809(1):81-9. Inhibition of one of the primary pumps of -driven ATP synthesis, the NADH:Q oxidoreductase, with rotenone also resulted in an inhibition of the rate of ATP synthesis proportional to that of the oxidation. |
2(0,0,0,2) | Details |
| 2972256 | Hammerstedt RH, Volonte C, Racker E: Motility, heat, and lactate production in ejaculated bovine sperm. . Arch Biochem Biophys. 1988 Oct;266(1):111-23. Effects of various inhibitors on motility, heat, and lactate production of ejaculated bovine sperm were determined in the presence of antimycin A and rotenone. erythro-9-[3-(2-Hydroxynonyl)] (EHNA) and polyvinylpyrrolidone (PVP-360) stopped motility and reduced heat or lactate production by 30-50%. did, however, inhibit ATPase activity of plasma membrane, suggesting that this unidentified ATPase may contribute to the formation of ADP and Pi required for lactate production by the intact cell. |
2(0,0,0,2) | Details |
| 10727606 | Guzman-Grenfell AM, Bonilla-Hernandez MA, Gonzalez-Martinez MT: induces a Na (+),K (+)-ATPase-dependent transient hyperpolarization in human sperm. Biochim Biophys Acta. 2000 Apr 5;1464(2):188-98. The hyperpolarization was inhibited by NaF, a glycolysis inhibitor, but not by mitochondrial inhibitors rotenone and antimycin), suggesting that it depended on glycolysis. |
2(0,0,0,2) | Details |
| 562603 | Thurman RG, Brentzel HJ, McKenna WR: Common mechanism for the adaptive increase in hepatic and metabolism due to chronic pretreatment with Adv Exp Med Biol. 1977;85A:237-45. uptake in both -treated and control livers was similarly sensitive to inhibition by 4-methylpyrazole, rotenone, and antimycin A. The adaptive increase in uptake was apparently specifically abolished by ouabain, an inhibitor of the -plus -activated ATPase. |
2(0,0,0,2) | Details |
| 19715769 | Panfoli I, Calzia D, Bianchini P, Ravera S, Diaspro A, Candiano G, Bachi A, Monticone M, Aluigi MG, Barabino S, Calabria G, Rolando M, Tacchetti C, Morelli A, Pepe IM: Evidence for aerobic metabolism in retinal rod outer segment disks. Int J Biochem Cell Biol. 2009 Dec;41(12):2555-65. Epub 2009 Aug 26. In a previous proteomic study we reported the expression of the respiratory chain complexes I-IV and the oxidative phosphorylation Complex V (F (1) F (0)-ATP synthase) in disks. An consumption, stimulated by and and reverted by rotenone, antimycin A and KCN was measured in disks, either in coupled or uncoupled conditions. |
1(0,0,0,1) | Details |
| 2450579 | Petronilli V, Azzone GF, Pietrobon D: Analysis of mechanisms of free-energy coupling and uncoupling by inhibitor titrations: theory, computer modeling and experiments. Biochim Biophys Acta. 1988 Mar 9;932(3):306-24. The rates of ATP synthesis and of ATP-driven NAD reduction have been measured in bovine heart submitochondrial particles as a function of the fraction of inhibited redox pumps (in titrations with either antimycin or rotenone) and of the fraction of inhibited ATPases (in titrations with DCCD). The flux control coefficients of the redox and ATPase proton pumps on the rates of ATP synthesis and of ATP-driven NAD reduction have been derived and found to be equal to 1 for both pumps; i.e., both pumps appear to be 'completely rate limiting'. |
1(0,0,0,1) | Details |
| 4262959 | Margreth A, Salviati G, Di Mauro S, Turati G: Early biochemical consequences of denervation in fast and slow skeletal muscles and their relationship to neural control over muscle differentiation. Biochem J. 1972 Mar;126(5):1099-110. The isolated sarcoplasmic reticulum from fast muscle showed a slight diminution of ATPase-linked Ca (2+)-transport activity and a selective increase of rotenone-insensitive -cytochrome c reductase activity, in addition to a greater emphasis on slow-type electrophoretic components of the structural membrane protein. 7. |
83(1,1,1,3) | Details |
| 15355852 | Humez S, Monet M, van Coppenolle F, Delcourt P, Prevarskaya N: The role of intracellular pH in cell growth arrest induced by ATP. Am J Physiol Cell Physiol. 2004 Dec;287(6):C1733-46. Epub 2004 Sep 8. ATP-induced acidification was reduced after the dissipation of the mitochondrial gradient by rotenone and carbonyl p-trifluoromethoxyphenylhydrazone, after inhibition of Ca (2+) uptake into the mitochondria by ruthenium red, and after inhibition of the F (0) F (1)-ATPase with oligomycin. |
81(1,1,1,1) | Details |
| 7578568 | Dedov VN, Gabai VL, Cherniak BV: [Effect of protein inhibitors of mitochondrial ATPase in intact rat thymocytes and Ehrlich ascites carcinoma cells]. Biokhimiia. 1995 Jul;60(7):1138-45. It has been found that inhibition of mitochondrial ATPase in living thymocytes and Ehrlich ascites carcinoma (EAC) cells after incubation of cells with uncoupler, rotenone or cumene hydroperoxide, depends, in a large measure, on the inhibitor protein (IF1) action. Maximum inhibition (up to 70% of the oligomycin-sensitive ATPase activity) was found in the presence of the uncoupler in the incubation medium. |
2(0,0,0,2) | Details |
| 10947970 | Roussel D, Lhenry F, Ecochard L, Sempore B, Rouanet JL, Favier R: Differential effects of endurance training and depletion on regional mitochondrial adaptations in rat skeletal muscle. Biochem J. 2000 Sep 1;350 Pt 2:547-53. Mitochondrial oxidative phosphorylation was assessed polarographically by using plus (plus rotenone), and plus N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) (plus antimycin) as respiratory substrates. Assays of cytochrome c oxidase and F (1)-ATPase activities were also performed. |
2(0,0,0,2) | Details |
| 6086170 | Kramer JH, Mak IT, Weglicki WB: Differential sensitivity of canine cardiac sarcolemmal and microsomal enzymes to inhibition by free radical-induced lipid peroxidation. Circ Res. 1984 Jul;55(1):120-4. Sarcolemmal and microsomal membranes prepared from adult canine cardiac myocytes (sarcolemmal Na+, K+-ATPase = 71.8 mumol/mg per hr and microsomal rotenone-insensitive cytochrome c reductase = 114 mumol/mg per hr) were each preincubated at 37 degrees C in the presence of a free radical generating system consisting of and Fe - loss of the Na+, K+-ATPase and reductase activities, as well as the associated increases in lipid peroxidation, measured by malondialdehyde formation, were temporally correlated in both systems. The ATPase was inhibited 70% when the malondialdehyde was 71 nmol/mg protein at 20 minutes and 90% when malondialdehyde was 138 nmol/mg protein at 90 minutes. |
1(0,0,0,1) | Details |
| 1260063 | Babitch JA, Breithaupt TB, Chiu TC, Garadi R, Helseth DL: Preparation of chick brain synaptosomes and synaptosomal membranes. . Biochim Biophys Acta. 1976 Apr 16;433(1):75-89. Purity of the subcellular and subsynaptosomal fractions was monitored by electron microscopy and measurements of ferrocytochrome c: oxidoreductase (EC 1.9.3.)), monoamine: oxidoreductase (deaminating) EC 1.4.3.4), rotenone-insensitive cytochrome c oxidoreductase (EC 1.6.99.3), cytochrome c oxidoreductase (EC 1.6.99.1), orthophosphoric monoester phosphohydrolase (EC 3.1.3.2), ATP phosphohydrolase (EC 3.6.1.4), and levels of RNA. Levels of this enzyme in the membrane fraction are enriched 13-fold over homogenate ATPase levels. |
1(0,0,0,1) | Details |
| 11311802 | Dedov VN, Mandadi S, Armati PJ, Verkhratsky A: -induced depolarisation of mitochondria in dorsal root ganglion neurons is enhanced by vanilloid receptors. Neuroscience. 2001;103(1):219-26. Rotenone, an inhibitor of complex I of the mitochondrial respiratory chain, and oligomycin, an inhibitor of F (0) F (1)-ATPase, significantly enhanced the mitochondrial depolarisation produced by in DRG neurons. Rotenone, an inhibitor of complex I of the mitochondrial respiratory chain, and oligomycin, an inhibitor of F (0) F (1)-ATPase, significantly enhanced the mitochondrial depolarisation produced by in DRG neurons. |
1(0,0,0,1) | Details |
| 2831981 | Kier AB, Parker MT, Schroeder F: Local and metastatic tumor growth and membrane properties of LM fibroblasts in athymic (nude) mice. Biochim Biophys Acta. 1988 Mar 3;938(3):434-46. When compared to the -fed tumor cells, the specific activities of three mitochondrial enzymes, namely dependent, rotenone insensitive -dependent, and rotenone sensitive -dependent cytochrome-c reductase, were significantly increased in the analogue-supplemented cells. As compared to local tumor plasma membranes, the lung metastasis plasma membranes had elevated (Na+ + K+)-ATPase specific activity, phospholipid oleic and content, and fluidity. |
1(0,0,0,1) | Details |
| 16668682 | Soole KL, Dry IB, Wiskich JT: Partial Purification and Characterization of Complex I, NADH:Ubiquinone Reductase, from the Inner Membrane of Beetroot Mitochondria. Plant Physiol. 1992 Feb;98(2):588-594. The NADH dehydrogenase preparation contained a minor ATPase contamination. The purified NADH dehydrogenase complex catalyzed the reduction of various electron acceptors with as the electron donor, was not sensitive to rotenone inhibition, and had a slow - reductase activity. |
1(0,0,0,1) | Details |
| 7476912 | Garcia-Ruiz C, Colell A, Morales A, Kaplowitz N, Fernandez-Checa JC: Role of oxidative stress generated from the mitochondrial electron transport chain and mitochondrial status in loss of mitochondrial function and activation of transcription factor nuclear factor-kappa B: studies with isolated mitochondria and rat hepatocytes. Mol Pharmacol. 1995 Nov;48(5):825-34. However, blockade of complex I or II with rotenone or thenoyltrifluoroacetone, respectively, did not result in accumulation of peroxide. The endogenous oxidative stress induced by AA was accompanied by a moderate loss of activity of ATPase complex (77% of control) and complex IV of respiration (75% of control), which was accentuated after depletion of mitochondrial GSH (51% and 45% of control, respectively). |
1(0,0,0,1) | Details |
| 11874555 | Vanden Berghe P, Missiaen L, Janssens J, Tack J: signalling and removal mechanisms in myenteric neurones. Neurogastroenterol Motil. 2002 Feb;14(1):63-73. To characterize further the Ca2+ signalling mechanisms of myenteric neurones, we studied the effect of thapsigargin, a blocker of the Ca2+-store ATPase, and the mechanisms involved in restoring the intracellular Ca2+ concentration ([Ca2+] i) after activation. Mitochondrial blockers (rotenone and antimycin/oligomycin) also prolonged the responses, but without affecting the amplitude. |
1(0,0,0,1) | Details |
| 8476414 | Tatuch Y, Robinson BH: The mitochondrial DNA mutation at 8993 associated with NARP slows the rate of synthesis in isolated lymphoblast mitochondria. Biochem Biophys Res Commun. 1993 Apr 15;192(1):124-8. Rates of synthesis with / /rotenone, /N'N'N'N' tetramethyl phenylene diamine were reduced to 67%, 58% and 54% of the control rates, respectively. The backward reaction measured as oligomycin sensitive ATPase was reduced to an average of 42% of that in controls. |
1(0,0,0,1) | Details |
| 16608844 | de Meis L, Arruda AP, da Costa RM, Benchimol M: Identification of a Ca2+-ATPase in brown adipose tissue mitochondria: regulation of thermogenesis by ATP and Ca2+. J Biol Chem. 2006 Jun 16;281(24):16384-90. Epub 2006 Apr 11. Heat production was partially inhibited by the ionophore carbonyl p-trifluoromethoxyphenylhydrazone and abolished by thapsigargin, a specific ER Ca (2+)-ATPase inhibitor, and by both rotenone and KCN, two substances that inhibit the electron transfer trough the mitochondrial cytochrome chain. |
36(0,1,1,6) | Details |
| 123770 | Bertina RM, Slater EC: The effects of and electron transport on the carbonyl m-chlorophenylhydrazone-induced ATPase of rat-liver mitochondria. Biochim Biophys Acta. 1975 Mar 20;376(3):492-504. The inhibitory action of respiratory-chain inhibitors on the ATPase activity, which is independent of the actual inhibitor used, is greatly delayed or prevented by the presence of uncoupler, and, in the case of rotenone, can be reversed completely by the subsequent addition of (in the absence of uncoupler). |
36(0,1,1,6) | Details |
| 224923 | Bers DM: Isolation and characterization of cardiac sarcolemma. Biochim Biophys Acta. 1979 Jul 19;555(1):131-46. These vesicles are enriched about ten-fold (with respect to the tissue homogenate) in K+-stimulated p-nitrophenylphosphatase, (Na+ + K+)-ATPase, 5'-nucleotidase activities and content, all of which are believed to be components of the sarcolemma. There may, however, be some contamination by outer mitochondrial membranes (as judged by monoamine oxidase and rotenone-insensitive cytochrome c reductase activities) which have rarely been monitored in cardiac sarcolemmal preparations. |
2(0,0,0,2) | Details |
| 10399318 | Gaskova D, Brodska B, Holoubek A, Sigler K: Factors and processes involved in membrane potential build-up in yeast: diS-C3 (3) assay. Int J Biochem Cell Biol. 1999 May;31(5):575-84. The strongly pHout-dependent depolarisation caused by the protonophores CCCP and FCCP, lack of effect of the respiratory chain inhibitors rotenone and HQNO on the delta psi, as well as results obtained with a respiration-deficient rho- mutant show that the major component of the diS-C3 (3)-reported membrane potential is the delta psi formed on the plasma membrane while mitochondrial potential forms a minor part of the delta psi. Its role may be reflected in the slight depolarisation caused by the F1F0-ATPase inhibitor azide in both rho- mutant and wildtype cells. |
2(0,0,0,2) | Details |
| 19772851 | Laberge RM, Ambadipudi R, Georges E: P-glycoprotein (ABCB1) modulates collateral sensitivity of a multidrug resistant cell line to Arch Biochem Biophys. 2009 Nov;491(1-2):53-60. Epub 2009 Sep 20. We previously demonstrated that collateral sensitivity to correlates with the effect of this drug on P-gp1 ATPase, and is reversed by inhibitors of P-gp1 ATPase (e.g., PSC 833 and Ivermectin). Moreover, known inhibitors of ETC, rotenone and antimycin A which cause an increase in reactive species, synergized with -induced collateral sensitivity leading to increased cell death as determined by MTT cell survival assay. |
1(0,0,0,1) | Details |
| 1534202 | Noll T, Koop A, Piper HM: Mitochondrial ATP-synthase activity in cardiomyocytes after aerobic-anaerobic metabolic transition. Am J Physiol. 1992 May;262(5 Pt 1):C1297-303. The inhibitor of the mitochondrial F1,F0- ATPase oligomycin, however, added upon establishment of hypoxia, did not slow down, as in the case of depolarized mitochondria, but moderately accelerated energy depletion. 2) Activation of mitochondrial ATP hydrolysis could be provoked in these hypoxic cells by addition of antimycin A, and rotenone, i.e., specific inhibitors of certain sites of the respiratory chain. It has been hypothesized that, in -depleted myocardial cells, mitochondria are depolarized and the F1,F0- adenosinetriphosphatase (ATPase) catalyzes net ATP hydrolysis when the cells exhibit the signs of an aerobic-anaerobic metabolic transition, which are increased lactate formation and decline in high-energy reserves [W. |
1(0,0,0,1) | Details |
| 1939077 | Fasolato C, Zottini M, Clementi E, Zacchetti D, Meldolesi J, Pozzan T: Intracellular Ca2+ pools in PC12 cells. J Biol Chem. 1991 Oct 25;266(30):20159-67. Chem. 266, 20152-20158) accounts for approximately equal to 200 microM of Ca2+/liter of cell water (less than 30% of total exchangeable Ca2+) and takes up Ca2+ from the cytosol via a Ca (2+)-ATPase, blocked by thapsigargin. |
1(0,0,0,1) | Details |
| 612130 | Radeva-Domushieva D, Russanov E: Effect of single exhaustive swimming on mitochondrial enzyme activities in rat myocardium. Acta Physiol Pharmacol Bulg. 1977;3(3):49-55. In rat heart mitochondria the activities of SDH, cytochrome oxidase and ATPase (DNP-stimulated) increase after swimming and remain at that level until the end of the 22-hour rest period studied. The marker enzyme of the outer mitochondrial membranes--rotenone-insensitive NAD.H-cytochrome c-reductase--reveals unchanged activity after swimming and a 22-hour period of rest. |
1(0,0,0,1) | Details |
| 5972170 | Bittar EE: Effect of inhibitors and uncouplers on the Na pump of the Maia muscle fibre. J Physiol. 1966 Nov;187(1):81-103. The inhibitory state due to externally applied ethacrynic acid is shown to be sensitive to 40 mM-K (+) in the bathing medium.(ii) Internally applied chlorpromazine causes 40% inhibition.(iii) Internally applied causes a slight increase or a slight decrease in Na efflux or no effect.(iv) Internally applied antimycin A produces a net inhibitory effect of almost 50%.(v) Internally applied rotenone produces a 60% inhibitory effect while fibres that fail to contract initially show about a 90% fall in efflux.(vi) The response pattern to internally applied oligomycin is similar to that of antimycin but the net inhibitory effect is only 25%.(vii) Internally applied atractylate produces 45% inhibition.(viii) Internally applied gramicidin A produces about 40% inhibition.(ix) Internally applied valinomycin is without an effect, but in the presence of 40 mM-K (+) in the external medium there is marked inhibition of Na efflux.3. The evidence put forward suggests that the Na pump of the Maia fibre is divided into two parts, one the Na (+)-K (+)-ATPase, driven by ATP and (ArP), and the other, an electrogenic mechanism, driven by respiration and possibly direct coupling of glycolytic energy. |
1(0,0,0,1) | Details |
| 8294484 | Herz U, Schroder W, Liddell A, Leaver CJ, Brennicke A, Grohmann L: Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum. J Biol Chem. 1994 Jan 21;269(3):2263-9. The preparation was found to be virtually free of any ATPase or transhydrogenase contamination. The enzyme preparation showed an NADH:ubiquinone-2 reductase activity of 11.5 mumol x min-1 x mg-1 and is strongly inhibited by rotenone. |
1(0,0,0,1) | Details |
| 7607228 | Wisniewski E, Gellerich FN, Kunz WS: Distribution of flux control among the enzymes of mitochondrial oxidative phosphorylation in -activated saponin-skinned rat musculus soleus fibers. Eur J Biochem. 1995 Jun 1;230(2):549-54. The flux control coefficients of H (+)-ATPase, -nucleotide translocase, transporter, NADH:ubiquinone oxidoreductase and cytochrome-c oxidase were determined to be equal to 0.16 +/- 0.08 (n = 6), 0.34 +/- 0.12 (n = 5), 0.08 +/- 0.03 (n = 5), 0.01 +/- 0.006 (n = 4) and 0.09 +/- 0.03 (n = 3) using inhibitor titrations with the specific inhibitors oligomycin, carboxyatractyloside, mersalyl, rotenone and respectively, and applying non-linear regression of the entire titration curve. |
35(0,1,1,5) | Details |
| 2894857 | Sambasivarao D, Kramer R, Rao NM, Sitaramam V: hydrolysis induces variable porosity to in the mitochondrial inner membrane. Biochim Biophys Acta. 1988 Mar 30;933(1):200-11. Osmotic titration of ATPase activity in rat liver mitochondria was consistent with enhanced porosity of the mitochondrial inner membrane to due to hydrolysis even when endogenous respiration was inhibited by rotenone. |
32(0,1,1,2) | Details |
| 12003774 | Sakurai E, Sakurada T, Ochiai Y, Yamakami J, Tanaka Y: Stereoselective transport of in rat lung microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2002 Jun;282(6):L1192-7. The addition of metabolic inhibitors [2,4-dinitrophenol (DNP) and rotenone] reduced the uptake rate of Ouabain, an inhibitor of Na (+)-K (+)-ATPase, also reduced uptake of |
1(0,0,0,1) | Details |
| 6787824 | Bohme G, Schonfeld P, Kuster U, Kunz W, Lyr H: The multifunctional actions of beta-thujaplicin on the oxidative energy transformations as a consequence of its lipophilic and chelating properties. Acta Biol Med Ger. 1980;39(11-12):1153-63. The influence of the fungicidic compound beta-thujaplicin (beta-isopropyl-tropolone) on the energy transformation processes of oxidative phosphorylation was investigated in isolated rat liver mitochondria with (plus rotenone) as substrate. However, the effective suppression of the DNP-induced ATPase by beta-thujaplicin explains the pronounced inhibition of active respiration. |
1(0,0,0,1) | Details |
| 16328369 | Park JP, Fioravanti CF: Catalysis of --> transhydrogenation by adult Hymenolepis diminuta mitochondria. Parasitol Res. 2006 Feb;98(3):200-6. Epub 2005 Dec 3. Oxidase inhibition by rotenone was unaffected by BSA. Although DCCD inhibited oxidase and ATPase, BSA only lessened oxidase inhibition. |
1(0,0,0,1) | Details |
| 20209153 | de Meis L, Ketzer LA, da Costa RM, de Andrade IR, Benchimol M: Fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue: activation of thermogenesis by Ca2+. PLoS One. 2010 Mar 2;5(3):e9439. Ca (2+)-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. |
1(0,0,0,1) | Details |
| 219869 | Zimmer G, Ohlenschlager G, Berger J: [Biochemical and clinical studies on the influence of 2-mercaptopropionylglycine on liver cell function (author's transl)]. Arzneimittelforschung. 1978;28(6):961-70. The ATPase activity in mitochondria was diminished, while ATP-contents of the mitochondrial suspensions were increased by the drug. Reversal of electron transport with formation of by in the presence of rotenone and ATP was, concentration dependently, improved by MPG. |
1(0,0,0,1) | Details |
| 6087900 | Doussiere J, Ligeti E, Brandolin G, Vignais PV: Control of oxidative phosphorylation in rat heart mitochondria. Biochim Biophys Acta. 1984 Aug 31;766(2):492-500. As shown by carboxyatractyloside titration curves under different conditions, the relative importance of the nucleotide carrier depends on the mode of regeneration (F1-ATPase or plus hexokinase) of ADP from ATP exported outside mitochondria, on the total concentration of nucleotides present in the medium and on the mode of limitation of the rate of respiration rotenone, oligomycin or mersalyl). |
31(0,1,1,1) | Details |
| 1760526 | Arregui MP, Perez-Martos A, Lopez-Perez MJ: Detection of barbiturate-induced surface changes in cerebrocortical synaptosomes by phase partitioning in an aqueous two-phase system. Biosci Rep. 1991 Aug;11(4):181-8. Dithionite-induced anoxia, rotenone and ouabain also induced a similar increase of partition of synaptosomes into the upper phase, suggesting that the surface changes detected by phase partitioning modification of synaptosomes could be somehow related to the bioenergetic maintenance of the membrane ATPase. |
31(0,1,1,1) | Details |
| 2839496 | Connolly E, Nedergaard J: Beta-adrenergic modulation of Ca2+ uptake by isolated brown adipocytes. J Biol Chem. 1988 Aug 5;263(22):10574-82. The artificial mitochondrial uncoupler carbonyl p-trifluoromethoxyphenylhydrazone (FCCP) induced a large reduction in cellular Ca2+ uptake (even in the presence of the ATPase inhibitor oligomycin), and in the presence of FCCP the inhibitory effect of on Ca2+ uptake was significantly reduced. The effect of beta-adrenergic stimulation on Ca2+ uptake was not directly caused by the large increase in respiration that occurs in response to because the respiratory inhibitor rotenone did not affect the Ca2+ response of the cells to the hormone. |
1(0,0,0,1) | Details |
| 1645458 | Bienen EJ, Saric M, Pollakis G, Grady RW, Clarkson AB Jr: Mitochondrial development in Trypanosoma brucei brucei transitional bloodstream forms. Mol Biochem Parasitol. 1991 Apr;45(2):185-92. The EMF is inhibited by 2,4-dinitrophenol, rotenone and salicylhydroxamic acid but not by antimycin A or This putative production is either by F1F0 ATPase driven by the complex I proton pump or by mitochondrial substrate level phosphorylation, or most likely by both. |
1(0,0,0,1) | Details |
| 1666984 | Iossa S, Barletta A, Liverini G: Hepatic selective adjustments in short-term cold exposed rats. Cell Biochem Funct. 1991 Oct;9(4):275-80. In addition, we have measured plasma membrane Na+/K (+)-ATPase activity. |
1(0,0,0,1) | Details |
| 7592589 | Sanchez-Alcazar JA, Hernandez I, De la Torre MP, Garcia I, Santiago E, Munoz-Yague MT, Solis-Herruzo JA: Down-regulation of tumor necrosis factor receptors by blockade of mitochondrial respiration. J Biol Chem. 1995 Oct 13;270(41):23944-50. This effect was dose- and time-related and was observed independently of the level at which the respiration was blocked (respiratory chain, backflow, ATPase, anaerobiosis). |
1(0,0,0,1) | Details |
| 11781140 | Gonzalez A, Schmid A, Salido GM, Camello PJ, Pariente JA: XOD-catalyzed ROS generation mobilizes from intracellular stores in mouse pancreatic acinar cells. Cell Signal. 2002 Feb;14(2):153-9. ROS still released Ca (2+) when the endoplasmic reticulum Ca (2+)-ATPase was blocked with thapsigargin (1 microM), or when rotenone (10 microM) was added to release Ca (2+) from mitochondria. |
31(0,1,1,1) | Details |
| 10841308 | de Halac IN, Bacman SR, de Kremer RD: Histoenzymology of oxidases and dehydrogenases in peripheral blood lymphocytes and monocytes for the study of mitochondrial oxidative phosphorylation. Histochem J. 2000 Mar;32(3):133-7. The specificity of each histoenzymological reaction was tested using a specific respiratory chain inhibitor: rotenone for diaphorase, thenoyltrifluoroacetone for succinate dehydrogenase, for cytochrome c oxidase and oligomycin for ATPase. |
31(0,1,1,1) | Details |
| 18439569 | Lopez JJ, Jardin I, Bobe R, Pariente JA, Enouf J, Salido GM, Rosado JA: STIM1 regulates acidic Ca2+ store refilling by interaction with SERCA3 in human platelets. Biochem Pharmacol. 2008 Jun 1;75(11):2157-64. Epub 2008 Mar 22. Platelets possess agonist-releasable Ca (2+) stores in acidic organelles where sarcoendoplasmic reticulum Ca (2+)-ATPase-3 (SERCA) pump is involved in store refilling. |
1(0,0,0,1) | Details |
| 237916 | Thayer WS, Hinkle PC: Synthesis of by an artificially imposed electrochemical gradient in bovine heart submitochondrial particles. J Biol Chem. 1975 Jul 25;250(14):5330-5. These results demonstrate that an electrochemical gradient of protons can drive the synthesis of ATP by reversal of the -translocating ATPase independent of electron transport. |
1(0,0,0,1) | Details |
| 2125381 | Karamushka VI, Gruzina TG, Ul'berg ZR: [The effect of respiratory toxins on the bacterial concentration of trivalent gold]. Ukr Biokhim Zh. 1990 Sep-Oct;62(5):103-6. It has been found that concentration of trivalent gold by Bacillus cereus V-4368 cells is sensitive to respiratory inhibitors such as rotenone and antimycin A. It is supposed that Au (III) concentration is due to the ATPase action and the respiration systems supply this process with |
1(0,0,0,1) | Details |
| 2833248 | Missiaen L, Wuytack F, Casteels R: Characterization of the Mg2+-activated ATPase activity in smooth-muscle membranes. Biochem J. 1988 Mar 1;250(2):579-88. The apparent initial non-linearity of the Mg2+-ATPase activity in that tissue is due to a time-dependent decrease of a rotenone-sensitive oxidase activity. |
15(0,0,1,10) | Details |
| 2833420 | Mitsunaga K, Fujino Y, Yasumasu I: Distributions of H+,K+-ATPase and Cl-,HCO3 (-)-ATPase in micromere-derived cells of sea urchin embryos. Differentiation. 1987;35(3):190-6. Considerable activity of rotenone-insensitive -cytochrome c reductase, a marker enzyme for microsome, was detectable in the microsome fraction. |
7(0,0,0,7) | Details |
| 16828729 | Benit P, Goncalves S, Philippe Dassa E, Briere JJ, Martin G, Rustin P: Three spectrophotometric assays for the measurement of the five respiratory chain complexes in minuscule biological samples. Clin Chim Acta. 2006 Dec;374(1-2):81-6. Epub 2006 Jun 2. A third assay measures rotenone-sensitive complex I activity and subsequently oligomycin-sensitive complex V activity. |
6(0,0,1,1) | Details |
| 3085949 | Griffith TM, Edwards DH, Newby AC, Lewis MJ, Henderson AH: Production of endothelium derived relaxant factor is dependent on oxidative phosphorylation and extracellular Cardiovasc Res. 1986 Jan;20(1):7-12. Production of endothelium derived relaxant factor was rapidly inhibited by agents that inhibit mitochondrial electron transport or F1-ATPase, or which uncouple oxidative phosphorylation, but was only slowly impaired by inhibition of glycolysis. |
1(0,0,0,1) | Details |
| 6242755 | Breen GA: Genetics of the mammalian oxidative phosphorylation system: characterization of a new oligomycin-resistant Chinese hamster ovary cell line. Mol Cell Biol. 1982 Jul;2(7):772-81. In vitro assays indicated that there was approximately a 25-fold increase in the resistance of the mitochondrial ATPase to inhibition by oligomycin in Olir 2.2 cells, with little change in the total ATPase activity. Olir 2.2 cells were cross-resistant to other inhibitors of the mitochondrial ATPase (such as rutamycin, ossamycin, peliomycin, venturicidin, leucinostatin, and efrapeptin) and to other inhibitors of mitochondrial functions (such as chloramphenicol, rotenone, and antimycin). |
1(0,0,0,1) | Details |
| 6950390 | Teissie J, Knox BE, Tsong TY, Wehrle J: Synthesis of in respiration-inhibited submitochondrial particles induced by microsecond electric pulses. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7473-7. ATP synthesis was also blocked by inhibitors of the F (0) F (1) ATPase complex, oligomycin, N,N'-dicyclohexyl carbodiimide, venturicidin, and aurovertin. In all cases respiration was inhibited completely by using or rotenone. |
1(0,0,0,1) | Details |
| 123160 | Vignais PV, Vignais PM, Doussiere J: Functional relationship between the ADP/ATP-carrier and the F1-ATPase in mitochondria. Biochim Biophys Acta. 1975 Feb 17;376(2):219-30. Two types of mitochondria were used: 1, respiring mitochondria which were carrying out oxidative phosphorylation and which had been replenished in ATP by incubation in a medium supplemented with and 2, non-respiring mitochondria which had been partially depleted of ATP by incubation in a medium supplemented with rotenone and |
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| 12576057 | Gostimskaya IS, Grivennikova VG, Zharova TV, Bakeeva LE, Vinogradov AD: In situ assay of the intramitochondrial enzymes: use of alamethicin for permeabilization of mitochondria. Anal Biochem. 2003 Feb 1;313(1):46-52. Alamethicin-treated mitochondria show high rotenone-sensitive oxidase, -quinone reductase, and oligomycin-sensitive and carboxyatractylate-insensitive ATPase activities. |
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| 172130 | Wagner T, Rafael J: ATPase complex and oxidative phosphorylation in chloramphenicol-induced megamitochondria from mouse liver. Biochim Biophys Acta. 1975 Dec 11;408(3):284-96. |
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| 171520 | Ryrie IJ: Studies of the oligomycin-sensitive ATPase from yeast mitochondria. J Supramol Struct. 1975;3(3):242-7. |
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| 8670305 | Wojtczak L, Nikitina ER, Czyz A, Skulskii IA: Cuprous ions activate glibenclamide-sensitive potassium channel in liver mitochondria. Biochem Biophys Res Commun. 1996 Jun 14;223(2):468-73. In K (+)-containing media, Cu+ collapsed the mitochondrial membrane potential formed by operation of the respiratory chain with or tetramethyl p-phenylenediamine + as substrates or by the -pumping ATPase. |
1(0,0,0,1) | Details |
| 3159138 | Veselkina MN, Bulychev AG, Braun AD: [Effect of metabolic inhibitors on formed novocaine and neutral red segregation zones in frog erythrocytes]. Tsitologiia. 1985 Apr;27(4):433-9. The volume of granules diminished under the influence of a glycolytic inhibitor iodoacetate, uncouplers of oxidative phosphorylation (2,4-dinitrophenol and carbonyl trifluoromethoxyphenylhydrozone), and respiratory inhibitors (antimycin A and rotenone), as well as under the influence of cycloheximide - an inhibitor of protein synthesis. It has been found that all these compounds are inhibitors of ATPase activity of the isolated segregation zones. |
1(0,0,0,1) | Details |
| 6495407 | Petrenko AIu, Belous AM, Zhegunov GF: [Ca2+ release from rat liver mitochondria subjected to deep freezing] . Ukr Biokhim Zh. 1984 Jul-Aug;56(4):447-51. ATPase and NADH-dehydrogenase inhibitors when introduced into the medium simultaneously with mitochondria prevent the both processes, but only till Ca2+ release. |
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| 2943316 | Kitagawa T, Akamatsu Y: Control of membrane permeability by external ATP in mammalian cells: isolation of an ATP-resistant variant from Chinese hamster ovary cells. Biochim Biophys Acta. 1986 Aug 21;860(2):185-93. We previously demonstrated that in CHO-K1 cells an ATP-dependent permeability change was induced in the presence of a mitochondrial inhibitor (KCN or rotenone), a cytoskeleton-attacking agent (vinblastine) and a calmodulin antagonist (trifluoperazine). |
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| 999790 | Walton E, Gladstone GP: Factors affecting the susceptibility of staphylococci to killing by the cationic proteins from rabbit polymorphonuclear leucocytes: the effects of alteration of cellular energetics and of various iron compounds. Br J Exp Pathol. 1976 Oct;57(5):560-70. Agents such as amytal and rotenone, which cause a block at the beginning of the chain and would increase its oxidation level, enhance killing, whereas those causing a block in or at the end of the chain, such a 2-n-heptyl-4-hydroxyquinoline-N-oxide, and anaerobiosis, which would cause reduction of a part or whole of the chain, prevent killing. |
0(0,0,0,0) | Details |
| 6321463 | Grover AK, Kwan CY, Luchowski E, Daniel EE, Triggle DJ: Subcellular distribution of [3H] nitrendipine binding in smooth muscle. . J Biol Chem. 1984 Feb 25;259(4):2223-6. There was an excellent correlation between the distribution of [3H] nitrendipine binding determined at the nitrendipine concentrations of 0.138 and 1.38 nM, and the distribution of the plasma membrane markers K+-activated ouabain-sensitive p-nitrophenylphosphatase, 5'-nucleotidase, phosphodiesterase I, and Mg-ATPase, but not between the mitochondrial markers cytochrome c, oxidase, -dependent cytochrome c reductase, or rotenone-insensitive -dependent cytochrome c reductase or the putative endoplasmic reticulum marker -dependent cytochrome c reductase. |
0(0,0,0,0) | Details |
| 2421768 | Herweijer MA, Berden JA, Slater EC: Uncoupler-inhibitor titrations of ATP-driven reverse electron transfer in bovine submitochondrial particles provide evidence for direct interaction between ATPase and NADH:Q oxidoreductase. Biochim Biophys Acta. 1986 Apr 24;849(2):276-87. Contrary to this prediction several uncouplers (S13, SF6847, 2,4-dinitrophenol, valinomycin + nigericin) show an increase in uncoupling efficiency in ATP-driven reverse electron transfer (reversal) upon inhibition of the secondary pump in this reaction, the NADH:Q oxidoreductase, by rotenone. |
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| 6453587 | Tyler DD: The pathway of inorganic- efflux from isolated liver mitochondria during hydrolysis. Biochem J. 1980 Dec 15;192(3):821-8. When mitochondria treated with mersalyl were incubated in buffered solution, almost all the P (i) liberated was recovered in the suspending medium, unless inhibitors of P (i)-induced large-amplitude swelling such as EDTA, EGTA, antimycin, rotenone, nupercaine or Mg (2+) were added. Larger amounts of P (i) appeared in the suspending medium during ATPase activity, despite the presence of N-ethylmaleimide, when LiCl/EGTA was used as suspending medium compared with /EDTA. |
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| 8761491 | Buchanan SK, Walker JE: Large-scale chromatographic purification of F1F0-ATPase and complex I from bovine heart mitochondria. Biochem J. 1996 Aug 15;318 ( Pt 1):343-9. The complex I preparation contains all of the subunits identified in other preparations of the enzyme, and has rotenone-sensitive NADH:ubiquinone oxidoreductase and NADH:ferricyanide oxidoreductase activities. |
4(0,0,0,4) | Details |
| 33385 | Holz RW: Evidence that catecholamine transport into chromaffin vesicles is coupled to vesicle membrane potential. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5190-4. FCCP increases and NEM decreases vesicular membrane ATPase activity. Reserpine and rotenone inhibit catecholamine influx but have no effect on the H (+) electrochemical gradient; they probably act at a step before coupling to the membrane potential (or the H (+) electrochemical gradient). |
1(0,0,0,1) | Details |
| 6138103 | Kravtsov GM, Pokudin NI, Gulak PV, Orlov SN: [Mechanisms of transport in brain synaptosomes as affected by depolarization]. Biokhimiia. 1983 Aug;48(8):1249-55. The decrease of the intracellular concentration of Ca2+ in depolarized nerve endings is mainly caused by the calmodulin-dependent Ca,Mg-ATPase of synaptolemma and mitochondria. A comparison of the kinetic parameters of Ca-transport systems in brain microsomes and mitochondria and the data on extensive inhibition of 45Ca uptake by rotenone and oligomycin suggest that mitochondria play a key role in the control of intraterminal concentration of Ca2+ during nerve impulse conduction. |
1(0,0,0,1) | Details |
| 20089711 | Zoer B, Cogolludo AL, Perez-Vizcaino F, De Mey JG, Blanco CE, Villamor E: Hypoxia sensing in the fetal chicken femoral artery is mediated by the mitochondrial electron transport chain. Am J Physiol Regul Integr Comp Physiol. 2010 Apr;298(4):R1026-34. Epub 2010 Jan 20. Hypoxia-induced relaxation was abolished or significantly reduced by the mETC inhibitors rotenone (complex I), myxothiazol and antimycin A (complex III), and NaN (3) (complex IV). |
0(0,0,0,0) | Details |
| 10609554 | Moreno G, Nocentini S, Guggiari M, Salet C: Effects of the lipophilic biscation, bis-pyridinium oxime BP12, on bioenergetics and induction of permeability transition in isolated mitochondria. Biochem Pharmacol. 2000 Feb 1;59(3):261-6. Respiration in the absence of with plus rotenone as substrate was not affected after treatment with various concentrations of BP12 up to 10 microM, while the -stimulated respiration was slowed down, with a parallel decrease in synthesis. |
0(0,0,0,0) | Details |
| 14638429 | Palese LL, Gaballo A, Technikova-Dobrova Z, Labonia N, Abbrescia A, Scacco S, Micelli L, Papa S: Characterization of plasma membrane respiratory chain and ATPase in the actinomycete Nonomuraea sp. FEMS Microbiol Lett. 2003 Nov 21;228(2):233-9. The respiratory chain is made up of a rotenone-sensitive -quinone oxidoreductase, a four subunits aa3-type cytochrome c oxidase and a bc1 complex. |
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| 6448069 | De Gomez-Puyou MT, Gavilanes M, Gomez-Puyou A, Ernster L: Control of activity states of heart mitochondrial ATPase. Biochim Biophys Acta. 1980 Oct 3;592(3):396-405. The ATPase complex of submitochondrial particles exhibits activity transitions that are controlled by the natural ATPase inhibitor (Gomez-Puyou, A., Tuena de Gomez-Puyou, M. and Ernster, L. (1979) Biochim. |
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| 4258694 | Gomez-Puyou A, Sandoval F, De Gomez-Puyou MT, Pena A, Chavez E: Dependency of the 2,4-dinitrophenol stimulated ATPase activity on K + and respiration. Biochem Biophys Res Commun. 1971 Oct 1;45(1):104-11. |
1(0,0,0,1) | Details |
| 7356983 | Martens ME, Jankulovska L, Neymark MA, Lee CP: Impaired substrate utilization in mitochondria from strain 129 dystrophic mice. Biochim Biophys Acta. 1980 Feb 8;589(2):190-200. Mg2+-stimulated ATPase activity was higher in dystrophic muscle mitochondria than in controls, but basal and oligomycin-insensitive activities were virtually identical to those of controls. |
1(0,0,0,1) | Details |
| 9338086 | Castillo G, Orce G: Metabolic inhibition and transport in isolated toad skin. . Arch Physiol Biochem. 1995 May;103(2):149-59. Rotenone, an inhibitor of the electron-transport chain, significantly decreased SCC and G in the unstimulated skin. Ouabain, an inhibitor of Na+,K (+)-ATPase, decreased SCC in the -stimulated skin, without affecting G. 9. |
1(0,0,0,1) | Details |
| 184082 | Hansford RG: Studies on the effects of -SH: and ratios on the interconversion of active and inactive pyruvate dehydrogenase in isolated rat heart mitochondria. J Biol Chem. 1976 Sep 25;251(18):5483-9. The content of -SH and of suspensions of rat heart mitochondria was stabilized by the addition of and acetyl- in the presence of the respiratory inhibitor rotenone. The mitochondrial content of NAD+ and was similarly stabilized by the addition of and and the content of ADP and ATP was imposed by the addition of these nucleotides to the mitochondrial suspension, in the presence of uncoupling agent and oligomycin, to inhibit ATPase. |
1(0,0,0,1) | Details |
| 12812914 | Bouyer P, Zhou Y, Boron WF: An increase in intracellular concentration that is induced by basolateral CO2 in rabbit renal proximal tubule. Am J Physiol Renal Physiol. 2003 Oct;285(4):F674-87. Epub 2003 Jun 17. Removing Ca2+ from the lumen and bath, or adding 10 microM nifedipine (L- and T-type Ca2+-channel blocker) or 2 microM thapsigargin [sarco-(endo) plasmic reticulum Ca2+-ATPase inhibitor] or 4 microM rotenone (mitochondrial inhibitor) to the lumen and bath, failed to reduce the CO2-induced increase in [Ca2+] i. |
0(0,0,0,0) | Details |
| 11766411 | Naito S, Nishimura M: Enantioselective uptake of BOF-4272, a xanthine oxidase inhibitor with a chiral sulfoxide, by isolated rat hepatocytes. Yakugaku Zasshi. 2001 Dec;121(12):989-94. Metabolic inhibitors such as antimycin, oligomycin, rotenone, carbonylcyanide m-chlorophenyl hydrazone, and carbonyl -p-(trifluromethoxy)-phenylhydrazone significantly inhibited uptake of the R (+) enantiomer, but had little effect on uptake of the S (-) enantiomer. |
0(0,0,0,0) | Details |
| 17006955 | Saunders R, Szymczyk KH, Shapiro IM, Adams CS: Matrix regulation of skeletal cell apoptosis III: mechanism of ion pair-induced apoptosis. J Cell Biochem. 2007 Feb 15;100(3):703-15. Inhibition of either the electron transfer chain (with antimycin a and rotenone), or the activation of a MMP transition (with bongkrekic acid) inhibited apoptosis in a dose-dependent manner. |
0(0,0,0,0) | Details |
| 6451185 | Rouslin W, Millard RW: Mitochondrial inner membrane enzyme defects in porcine myocardial ischemia. Am J Physiol. 1981 Feb;240(2):H308-13. After 2 h of occlusion, mitochondria from the ischemic area exhibited a 36 +/- 6% drop in state 3 respiratory activity (QO2) supported by the NAD-linked substrates, plus but only a 5 +/- 3% decrease in QO2 with plus rotenone. Mitochondrial ATPase (complex V) activity decreased by 48 +/- 2% with little change in its oligomycin sensitivity. |
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| 2145772 | Bidani A, Brown SE: ATP-dependent pHi recovery in lung macrophages: evidence for a plasma membrane H (+)-ATPase. Am J Physiol. 1990 Oct;259(4 Pt 1):C586-98. Parallel reductions in ATP content and the rate of pHi recovery were noted in the presence of antimycin A, rotenone, oligomycin, and iodoacetate. |
3(0,0,0,3) | Details |
| 3843927 | Briskin DP, Thornley WR, Poole RJ: Vanadate-dependent oxidation in microsomal membranes of sugar beet. Arch Biochem Biophys. 1985 Jan;236(1):228-37. While it is unclear as to the physiological significance of Na3VO4-dependent oxidation in plant cells, the presence of this system indicates that caution must be exercised when coupled ATPase assays depending upon oxidation are used with plant membranes in the presence of Na3VO4. The oxidation activity was insensitive to rotenone and antimycin A but was inhibited by NaN3, NaCN, and quinacrine. |
1(0,0,0,1) | Details |
| 11526446 | Smaili SS, Hsu YT, Sanders KM, Russell JT, Youle RJ: Bax translocation to mitochondria subsequent to a rapid loss of mitochondrial membrane potential. Cell Death Differ. 2001 Sep;8(9):909-20. The mitochondria uncoupler FCCP, in the presence of the F (1)-F (0) ATPase inhibitor oligomycin, can trigger Bax translocation to mitochondria suggesting that when ATP levels are maintained a collapse of DeltaPsi (m) induces Bax translocation. Bax association with mitochondria is also triggered by inhibitors of the electron transport chain, antimycin and rotenone, compounds that collapse DeltaPsi (m) without inducing rapid ATP hydrolysis that typically occurs with uncouplers such as FCCP. |
1(0,0,0,1) | Details |
| 16303854 | Zhou X, Ferraris JD, Burg MB: Mitochondrial reactive species contribute to high NaCl-induced activation of the transcription factor TonEBP/OREBP. Am J Physiol Renal Physiol. 2006 May;290(5):F1169-76. Epub 2005 Nov 22. We inhibited mitochondrial ROS production in HEK293 cells with rotenone and myxothiazol, which inhibit mitochondrial complexes I and III, respectively. |
0(0,0,0,0) | Details |
| 10717001 | Weinberg JM, Venkatachalam MA, Roeser NF, Nissim I: Mitochondrial dysfunction during hypoxia/reoxygenation and its correction by anaerobic metabolism of cycle intermediates. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2826-31. Rotenone, but not antimycin or oligomycin, prevented this effect, indicating that electron transport in complex I, rather than F (1) F (0)-ATPase activity, had been responsible for maintenance of DeltaPsi (m) by the substrates. |
0(0,0,0,0) | Details |
| 10958336 | Eder S, Benesic A, Freudinger R, Engert J, Schwerdt G, Drumm K, Gekle M: Nephritogenic ochratoxin A interferes with mitochondrial function and pH homeostasis in immortalized human kidney epithelial cells. Pflugers Arch. 2000 Aug;440(4):521-9. Inhibiting complexes I, III and IV of the mitochondrial electron transport chain (ETC) with rotenone, antimycin A and CN- prevented the OTA-induced acidification almost completely. |
0(0,0,0,0) | Details |
| 17084831 | Shinde SB, Save VC, Patil ND, Mishra KP, Tendolkar AG: Impairment of mitochondrial respiratory chain enzyme activities in tetralogy of Fallot. Clin Chim Acta. 2007 Feb;377(1-2):138-43. Epub 2006 Sep 26. The activities of rotenone-sensitive cytochrome c reductase (complexes I+III), cytochrome c oxidase (complex IV) and the ratio of I and III to II and III complexes (complex I) were significantly lower in TOF (p <0.001). Mitochondrial respiratory chain complexes, OXPHOS, cytochrome content and ATPase activity were measured by documented standard procedure. |
3(0,0,0,3) | Details |
| 16663354 | Marin B: Sensitivity of Tonoplast-Bound -Triphosphatase from Hevea to Inhibitors. Plant Physiol. 1983 Dec;73(4):973-977. The tonoplast-bound H (+)-translocating ATPase from Hevea latex was found to be insensitive to vanadate, diethylstilbestrol, and octylguanidine, which are specific inhibitors of the plasma membrane ATPase. The inhibitors of the mitochondrial ATPase, oligomycin and azide, and also rotenone and antimycin A, were all without effect. |
3(0,0,0,3) | Details |
| 14980383 | Barstow KL, Locknar SA, Merriam LA, Parsons RL: The modulation of action potential generation by -induced release is enhanced by mitochondrial inhibitors in mudpuppy parasympathetic neurons. Neuroscience. 2004;124(2):327-39. Exposure to the electron transport chain inhibitor rotenone (10 microM) alone or in combination with oligomycin (8 microg/ml) similarly increased the latency to AP generation. Exposure to the endoplasmic reticulum Ca-ATPase inhibitor thapsigargin (1 microM), decreased the latency to AP generation. |
1(0,0,0,1) | Details |
| 15843467 | Feldkamp T, Kribben A, Weinberg JM: F1FO-ATPase activity and ATP dependence of mitochondrial energization in proximal tubules after hypoxia/reoxygenation. J Am Soc Nephrol. 2005 Jun;16(6):1742-51. Epub 2005 Apr 20. Normoxic control tubules had a small ATP-dependent component to DeltaPsi (m), but it required low micromolar levels of ATP, not the millimolar levels needed to support DeltaPsi (m) in tubules de-energized with rotenone or after H/R. ATP hydrolysis by the oligomycin-sensitive, mitochondrial F (1) F (O)-ATPase was well preserved after H/R as long as Mg (2+) was available, indicating that function of both the F (1) F (O)-ATPase and of the nucleotide translocase, which delivers nucleotides to it, are largely intact. |
1(0,0,0,1) | Details |
| 6667089 | McEwan AG, Ferguson SJ, Jackson JB: Electron flow to dimethylsulphoxide or generates a membrane potential in Rhodopseudomonas capsulata. Arch Microbiol. 1983 Dec;136(4):300-5. The magnitude of the dimethylsulphoxide- or -dependent membrane potential was reduced either by a protonophore uncoupler of oxidative phosphorylation or synergistically by a combination of a protonophore plus rotenone, an inhibitor of electron flow from NADH dehydrogenase. |
0(0,0,0,0) | Details |
| 3214168 | Moore GA, Weis M, Orrenius S, O'Brien PJ: Role of sulfhydryl groups in -induced Ca2+ release by rat liver mitochondria. Arch Biochem Biophys. 1988 Dec;267(2):539-50. Incubation of rat liver mitochondria with derivatives in the presence of plus rotenone has been shown to cause NAD (P) H oxidation followed by Ca2+ release. |
0(0,0,0,0) | Details |
| 861265 | Tarshis MA, Fedotov NS: [Basic evidence for the active transport of carbohydrates in the membrane vesicles of Acholeplasma laidlawii cells]. Biofizika. 1977 Mar-Apr;22(2):275-80. transport is inhibited by anaerobiosis and by the electron transfer inhibitors such as rotenone and amytal, and by conductors such as carbonylcyanide-m-chlorophenylhydrazone. |
0(0,0,0,0) | Details |
| 8898917 | Rigoulet M, Devin A, Averet N, Vandais B, Guerin B: Mechanisms of inhibition and uncoupling of respiration in isolated rat liver mitochondria by the general anesthetic 2,6-diisopropylphenol. Eur J Biochem. 1996 Oct 1;241(1):280-5. We propose that ADP induces a change in conformation of ATPase, which leads to insensitivity of this complex towards diisopropylphenol. |
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| 2435377 | Chambers JP, Wayner MJ, Rizopoulos E, Gonzales ML, Taylor RB, Valdes JJ: Partial characterization of two (Ca+2 + Mg+2)-dependent ATPase activities from bovine brain synaptic membrane homogenates. Brain Res Bull. 1987 Jan;18(1):99-107. Only slight inhibition was observed in the presence of rotenone and oligomycin. |
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| 126982 | Hadler HI, Demetriou JM: The disturbance of oxidative phosphorylation by N-acetoxy-N-acetyl-2-aminofluorene, a model ultimate carcinogen. J Antibiot. 1975 Oct;28(10):809-18. The ATP energized decrease in absorbancy was inhibited by rutamycin, 2,4-dinitrophenol and a high level of antimycin known to induce ATPase activity. |
2(0,0,0,2) | Details |
| 10370869 | Zini R, Morin C, Bertelli A, Bertelli AA, Tillement JP: Effects of on the rat brain respiratory chain. Drugs Exp Clin Res. 1999;25(2-3):87-97. The rate of consumption by the different complexes was checked using rotenone (2 microM), (10 mM), antimycin A (1 microM), (KCN) (0.3 mM) and oligomycin (10 microM) to inhibit complexes II, III, IV, V and I, respectively. 5'- (ATP) synthase activity was checked as ATP hydrolysis (ATPase) at 37 degrees C for 10 min from purified mitochondria on Percoll gradient. |
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| 14676274 | Baker MA, Hetherington L, Ecroyd H, Roman SD, Aitken RJ: Analysis of the mechanism by which negatively regulates the phosphorylation cascade associated with sperm capacitation. J Cell Sci. 2004 Jan 15;117(Pt 2):211-22. Moreover, addition of the ATPase inhibitor thapsigargin, increased intracellular levels, decreased ATP and suppressed phosphorylation. In contrast, the mitochondrial inhibitor rotenone had no effect on either ATP levels or phosphorylation. |
1(0,0,0,1) | Details |
| 10989660 | Greenamyre JT, MacKenzie G, Peng TI, Stephans SE: Mitochondrial dysfunction in Parkinson's disease. Biochem Soc Symp. 1999;66:85-97. We developed a novel model of PD in which chronic, systemic infusion of rotenone, a complex-I inhibitor, selectively kills dopaminergic nerve terminals and causes retrograde degeneration of substantia nigra neurons over a period of months. Metabolic impairment depletes ATP, depresses Na+/K (+)-ATPase activity, and causes graded neuronal depolarization. |
1(0,0,0,1) | Details |
| 129171 | Dolgov AV, Ismanlov AD, Liakhovich VV, Severina II, Skulachev VP, Fateeva LA: [Respiration toxins as inhibitors of ion transport, supported by hydrolysis, in mitochondria]. Biokhimiia. 1975 Sep-Oct;40(5):934-41. The combination of three respiration inhibitors antimycin and rotenone) was shown to develope the following effects: 1) the inhibition of K+ accumulation by mitochondria at the presence of and valinomycin; 2) the decrease in acidification of non-mitochondrial space, accompanying to the K+ transport; 3) the activation of latent mitochondrial ATPase; 4) the inhibition of DNP-stimulated ATPase; 5) the inhibition of mitochondria swelling, caused by K+, Ca2+, or dimethyldibenzylammonium (DDA+) at the presence of ATP+phopshate (or 6) the stimulation of passive mitochondria swelling in 0.1 MNH4NO3; 7) the inhibition of -induced contraction of mitochondria, swelling in NH4NO3. |
31(0,1,1,1) | Details |
| 8531496 | Fitzgerald EM, Djamgoz MB: Sensitivity of valinomycin-based K (+)-selective micro-electrodes to inhibitors of K+ transport. J Neurosci Methods. 1995 Jul;59(2):273-7. The sensitivies of double-barrelled K (+)-selective micro-electrodes (KSMs) employing the low-impedance membrane cocktail based on the neutral K (+)-selective ion carrier valinomycin (Fluka, Cocktail B 60398) to the following 3 different classes of inhibitors of K+ transport were measured: (1) general metabolic inhibitors (dinitrophenol, azide, rotenone, dicyclohexylcarbodiimide, salicylhydroxamic acid); (2) P-type ATPase inhibitors (vanadate, ouabain, amiloride, SCH 28080); and (3) anion-dependent K+ transport inhibitors (bumetanide, 4-acetamide-4-isothiocyanostilbene-2,2-disulphonic acid). |
31(0,1,1,1) | Details |
| 2144730 | Tonsgard JH, Tung B, Kornafel KS, Getz GS: Environmentally induced differential amplification of mitochondrial populations. Biochem J. 1990 Sep 1;270(2):511-8. The TL mitochondrial -cytochrome c reductase is resistant to rotenone, whereas that of A9 mitochondria is sensitive to this agent. The ATPase of TL mitochondria is similarly resistant to rutamycin whether grown in the presence or absence of antibiotic. |
2(0,0,0,2) | Details |
| 8023940 | Del Castillo JR, Sulbaran-Carrasco MC, Burguillos L: K+ transport in isolated guinea pig colonocytes: evidence for Na (+)-independent ouabain-sensitive K+ pump. Am J Physiol. 1994 Jun;266(6 Pt 1):G1083-9. These transport mechanisms are sensitive to metabolic inhibition by rotenone and to vanadate, a blocker of type P adenosinetriphosphatase (ATPases). SCH-28080, an inhibitor of gastric K (+)-H (+)-ATPase, was without effect. |
2(0,0,0,2) | Details |
| 6454449 | Elbakidze GM: [Tissue-specific regulation of rat liver mitochondrial oxidative phosphorylation by a soluble phase of cells from that organ]. Biull Eksp Biol Med. 1981 Mar;91(3):315-7. SPC tissue-specifically inhibits ATPase activity of liver mitochondria. |
1(0,0,0,1) | Details |
| 7150743 | Zinchenko VP, Teplova VV, Evtodienko IuV: [Kinetics of consumption, luminescence of nucleotides and the cyanine dye 3',3'-diethylthiodicarbocyanine iodide after energizing Ehrlich ascites carcinoma cells with Biull Eksp Biol Med. 1982 Nov;94(11):69-72. Addition of to the cells in the presence of respiratory inhibitors similar to rotenon induces oscillations in the membrane mitochondrial potential due to H+-ATPase that uses glycolytic ATP. |
1(0,0,0,1) | Details |
| 17353442 | Kajimoto H, Hashimoto K, Bonnet SN, Haromy A, Harry G, Moudgil R, Nakanishi T, Rebeyka I, Thebaud B, Michelakis ED, Archer SL: activates the Rho/Rho-kinase pathway and induces RhoB and ROCK-1 expression in human and rabbit ductus arteriosus by increasing mitochondria-derived reactive species: a newly recognized mechanism for sustaining ductal constriction. Circulation. 2007 Apr 3;115(13):1777-88. Epub 2007 Mar 12. Activation of Rho-kinase in DA smooth muscle cells is initiated by a PO2-dependent, rotenone-sensitive increase in mitochondrion-derived reactive O2 species. Residual DA constriction reflects sensitization, which persists after removal of extracellular and blocking of sarcoplasmic reticulum Ca2+-ATPase. |
1(0,0,0,1) | Details |
| 7994564 | Chen CL, Sangiah S, Yu CA, Chen H, Berlin KD, Garrison GL, Scherlag BJ, Lazzara R: Effects of novel antiarrhythmic agents, BRB-I-28 and its derivatives, on the heart mitochondrial respiratory chain and sarcoplasmic reticulum Ca (2+)-ATPase. Res Commun Mol Pathol Pharmacol. 1994 Aug;85(2):193-208. The site of inhibition of BRB-I-28 and its derivatives on the respiratory chain was localized between flavoprotein n (FPn) and which is similar to the effect of rotenone and several other antiarrhythmic drugs such as amiodarone, etc. |
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| 6619770 | Working PK, Meizel S: Correlation of increased intraacrosomal pH with the hamster sperm acrosome reaction. J Exp Zool. 1983 Jul;227(1):97-107. Such sperm underwent a significant increase in the number of AR within 10 min after the addition of the Mg2+-ATPase triphosphatase) inhibitors DCCD (20 microM) or NBD-Cl (10 microM) or the ionophore FCCP (6 micrograms/ml) at 3.5 hr of incubation or after addition of HN4Cl (3 mM) at 4 hr of incubation. Addition of the mitochondrial electron transport inhibitor rotenone (2.5 microM) at 3.5 hr or of NaCl (3 mM) or KCl (3 mM) at 4 hr did not stimulate AR over control levels, suggesting that the stimulation of AR by the other compounds was not directly due to depletion of acrosomal (ATP) or alteration of the acrosomal transmembrane potential. |
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| 1752631 | Sitaramam V, Sambasivarao D: -dependent respiration in osmotically inactive swollen mitochondria: does transport replace phosphorylation in mediating respiratory control in swollen mitochondria?. Indian J Biochem Biophys. 1991 Aug;28(4):291-300. Rotenone-sensitive, uncoupler-insensitive, -dependent respiration was demonstrated in osmotically inactive fragments of the mitochondrial inner-membrane obtained following high amplitude (spontaneous) swelling. The thermodynamic relationship between respiratory control and stimulation of transport ATPase by the relevant transportable ligands could also be demonstrated in the conventional (rat liver) microsomes. |
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| 8276114 | Chernyak BV, Dedov VN, Gabai VL: Mitochondrial ATP hydrolysis and ATP depletion in thymocytes and Ehrlich ascites carcinoma cells. FEBS Lett. 1994 Jan 3;337(1):56-9. When Ehrlich ascites carcinoma (EAC) cells and thymocytes were treated with uncoupler or rotenone in -free medium, rapid ATP depletion was observed in both types of the cells. ATPase was blocked by the specific inhibitor protein (IF1) as it was shown by the studies on reactivation of this enzyme. |
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